Recombinant Mouse Anti-Human CHI3L1 Antibody (clone BU5B4)
CAT#: MOB-2482MZ
This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human CHI3L1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/77365/1774_G7_23_cr595293e11cc3a_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, HSkMC, THP-1, U-138 MG, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000133048-CHI3L1
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Specifications
- Immunogen
- Recombinant full length protein, corresponding to amino acids 22-383 of Human CHI3L1, purified from E coli
- Host Species
- Mouse
- Type
- Mouse antibody
- Specificity
- Human CHI3L1
- Species Reactivity
- Human
- Clone
- BU5B4
- Applications
- ELISA, IHC-P, WB
Target
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Specific CHI3L1 Staining
We used this antibody for immunohistochemistry on paraffin sections and the results were great. It specifically stained Human CHI3L1 without high background. The clone BU5B4 is very effective for tissue analysis.
Works In ELISA
In addition to IHC, we used this for ELISA quantification of the protein. It reacts well with the recombinant protein. It is a robust mouse monoclonal antibody that we rely on for our inflammation studies.
Q&As
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Is this antibody suitable for IHC on paraffin sections?
A: Yes, this recombinant mouse monoclonal antibody (clone BU5B4) specifically reacts with human CHI3L1 and is validated for Immunohistochemistry on paraffin-embedded sections (IHC-P), as well as ELISA and Western Blot.
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What immunogen was used to create this antibody?
A: This antibody was raised against a recombinant full-length protein corresponding to amino acids 22-383 of Human CHI3L1, purified from E. coli. It recognizes the Chitinase-3-like protein 1.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CHI3L1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3780z | Mouse Anti-CHI3L1 Recombinant Antibody (clone 42H3) | WB, ELISA | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4084 | Hi-Affi™ Rabbit Anti-CHI3L1 Recombinant Antibody (clone SI151DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0723-WK86 | Human Anti-CHI3L1 Recombinant Antibody (VS-0723-WK86) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1962 | Recombinant Rabbit Anti-Mouse CHI3L1 Capture Antibody (clone R79-6S-2) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1963 | Recombinant Rabbit Anti-Mouse CHI3L1 Detection Antibody (clone R34-4Z-7) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1964 | Recombinant Rabbit Anti-CHI3L1 Capture Antibody (clone R19-3L-2) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1436 | Anti-CHI3L1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1437 | Anti-Mouse CHI3L1 Immunohistochemistry Kit | IHC |
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