Recombinant Human Anti-human APOA1 Antibody Fab Fragment

CAT#: MHH-73-F(E)

Recombinant Human Antibody Fab Fragment recognizes and binds to Human APOA1, expressed in HEK 293 cells.

Gene Expression
Figure 1 IF staining of human cell line Hep G2 Figure 2 IHC staining of human endometrium Figure 3 IHC staining of human gallbladder Figure 4 IF staining of human cell line CACO-2 Figure 5 IF staining of human cell line U-2 OS Figure 6 RNA cell line category: Cell line enriched (Hep G2)

Specifications

  • Immunogen
  • Human apolipoprotein A-I
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Fab
  • Specificity
  • Tested positive against native Human apolipoprotein A-I
  • Species Reactivity
  • Human
  • Applications
  • Used for immunoassaytechniques such as: Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional Study

Product Property

  • Purity
  • >90% assessed by SDS-PAGE
  • Storage
  • Short Term Storage: 4°C
    Long Term Storage: -20°C

Target

  • Alternative Names
  • APOA1; apolipoprotein A-I; apo-AI
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Breast cancer biomarkers at key points during disease progression
Excellent for ELISA
I used the Recombinant Human Anti-human APOA1 Antibody (Fab Fragment) in my ELISA assays, and it worked wonderfully. The antibody provided clear and strong signals when detecting APOA1 in our samples. It was easy to use, and the instructions were straightforward. The results were consistent across multiple experiments, which helped us in our research on lipid metabolism. I am very satisfied with its performance and would recommend it to others.
Breast cancer biomarkers at key points during disease progression
High Specificity
The specificity of this antibody is impressive. It binds specifically to human APOA1 without cross-reacting with other proteins. In our experiments, we were able to detect APOA1 with confidence, which is essential for accurate data. The high specificity has greatly improved the reliability of our results. We are very pleased with this product and plan to continue using it.
Breast cancer biomarkers at key points during disease progression
Recommended for APOA1 Studies
I highly recommend this antibody to anyone studying APOA1. It has been a valuable tool in our lab for detecting and analyzing this important protein. The antibody performs reliably across different assays, and its specificity is excellent. It has helped us advance our research, and we will continue to use it in future projects.

Q&As

  1. What is the main target of the Recombinant Human Anti-human APOA1 Antibody (Fab Fragment)?

    A: The main target of this antibody is human Apolipoprotein A-I, often referred to as APOA1. APOA1 is a key protein component of high-density lipoproteins (HDL) in the blood, commonly known as "good cholesterol." It plays a vital role in transporting cholesterol from the body's tissues to the liver. This antibody specifically binds to APOA1, making it valuable for researchers studying cholesterol transport, lipid metabolism, and cardiovascular health in laboratory settings.

  2. Can this antibody be used in ELISA assays?

    A: Yes, this antibody works well in ELISA assays. It can detect and quantify the amount of APOA1 protein in your samples. By using it in ELISA, researchers can measure APOA1 levels in blood or cell extracts accurately. This is important for studying how APOA1 changes under different conditions and how it relates to cholesterol and lipid levels in research studies.

  3. Does this antibody recognize APOA1 from other species besides humans?

    A: This antibody is specifically designed to recognize human APOA1. While APOA1 is conserved across different species, cross-reactivity with APOA1 from other species like mouse or rat is not guaranteed. Researchers should verify cross-reactivity through experiments or consult the product datasheet or technical support for more information.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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