Recombinant Mouse Antibody binds selectively to Mouse FCGR2A, expressed in Raji.
Figure 1 Effect of FcgR blocking on ADCP.
Flow cytometry was used to calculate the percentage of tumour cell engulfment by cultured macrophages in the presence of 10 μg/ml of uhPR1A3. Fab2 fragments were used to block either FcgR I (CD64), FcgR II (CD32) or FcgR III (CD16) (each antibody concentration was1 mg ml1).
Ashraf, S., Q., Umana, P., & Mossner, et al. (2009). Humanised igg1 antibody variants targeting membrane-bound carcinoembryonic antigen by antibody-dependent cellular cytotoxicity and phagocytosis. BRITISH JOURNAL OF CANCER.
Figure 2 Expression of activating and inhibitory Fc Rs on human monocyte-derived DCs.
Surface expression of Fc RI (22.2-FITC) and Fc RII (IV.3-FITC and 7.3-FITC) on Ma, iDC, and mDC as assessed by flow cytometry.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Figure 3 Phagocytosis and TNF-α production mediated by activating and inhibitory Fc R in human DCs.
Phagocytosis of PKH26-labeled probes E-22.2, E-IV.3, and E-7.3 was measured by flow cytometry.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Figure 4 Phagocytosis and TNF-α production mediated by activating and inhibitory Fc R in human DCs.
Secretion of TNF-α by iDC cultured for 18 h E-IV.3 probes and E-7.3 probes as compared with E without Ab was assessed by ELISA.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Figure 5 Internalization of MP-coated Fc R-specific phagocytic probes by DCs.
Internalized MP following uptake of E-MP-7.3 by mDC was detected in immunofluorescence microscopy with rabbit anti-MP Abs and Alexa 546-conjugated goat antirabbit IgG counterstained with 7.3-FITC.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Figure 6 Differential expression of Fc R on DC alters cross-presentation of influenza MP to memory T cells.
ELISPOT assay for IFN-α-producing influenza memory T cells cultured with iDCs following uptake of 9-mer and 17-mer coupled to IV.3 probes (E-MP-IV.3) and 7.3 probes (E-MP-7.3).
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Figure 7 Cytokine regulation of Fc RII-mediated phagocytosis in mDCs.
Percent inhibition in the PI of E-7.3 probes compared with E-IV.3 probes measured in mDCs cultured in complete medium, IL-10, IL-13, and IL-13 plus IL-10.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-983 | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3); Fab Fragment | FC | Mouse Fab |
TAB-039WM-F(E) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); Fab Fragment | FC, WB, ELISA, Inhib | Human Fab |
HPAB-0223-YC-F(E) | Mouse Anti-FCGR2A Recombinant Antibody (clone mAb IV.3); Fab Fragment | ELISA, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-039WM-S(P) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); scFv Fragment | FC, WB, ELISA, Inhib | Human scFv |
HPAB-0223-YC-S(P) | Mouse Anti-FCGR2A Recombinant Antibody (clone mAb IV.3); scFv Fragment | ELISA, FuncS | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-790CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone IV.3) | Block | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-791CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone AT10) | Neut, FC, IHC-Fr, IP | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1272 | Hi-Affi™ Rabbit Anti-FCGR2A Recombinant Antibody (clone DS1272AB) | WB, IHC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-0295-CN | Rabbit Anti-FCGR2A Recombinant Antibody (clone JM10-70) | WB, IHC | Rabbit IgG |
FN-009CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3) | FC, Block | Mouse IgG1, κ |
VS3-XY686 | Mouse Anti-FCGR2A Recombinant Antibody, FITC (clone 4AIV-3) | FC, IF | Mouse IgG2b |
VS3-XY687 | Mouse Anti-FCGR2A Recombinant Antibody, PE (clone 4AIV-3) | FC, IF | Mouse IgG2b |
VS3-XY688 | Mouse Anti-FCGR2A Recombinant Antibody (clone 4AIV-3) | FC, IF | Mouse IgG2b |
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