Recombinant Mouse Anti-Human CD32 Antibody F(ab')2 Fragment, clone 7.3 (CAT#: DrMAB-982)
Recombinant Mouse Antibody binds selectively to Mouse FCGR2A, expressed in Raji.
Specific Inquiry
Block
Figure 1 Effect of FcgR blocking on ADCP.
Flow cytometry was used to calculate the percentage of tumour cell engulfment by cultured macrophages in the presence of 10 μg/ml of uhPR1A3. Fab2 fragments were used to block either FcgR I (CD64), FcgR II (CD32) or FcgR III (CD16) (each antibody concentration was1 mg ml1).
Ashraf, S., Q., Umana, P., & Mossner, et al. (2009). Humanised igg1 antibody variants targeting membrane-bound carcinoembryonic antigen by antibody-dependent cellular cytotoxicity and phagocytosis. BRITISH JOURNAL OF CANCER.
Specifications
- Immunogen
- Human K562 tumor cells and L cells transfected with Human Fc gamma RII
- Host Species
- Mouse
- Type
- F(ab')2
- Specificity
- Antibody reacts with a domain 2 epitope of all CD32 isoforms. It exhibits preferential binding to Fc gRIIb (inhibitory) isoform transfectants.
- Species Reactivity
- Human
- Clone
- 7.3
- Applications
- Block, FC, ELISA, IF, ELISPOT
Product Property
- Purification
- Protein A purification
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
- Concentration
- 1 mg/ml
- Buffer
- 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added
- Storage
- Store at 2-5°C. Open under aseptic conditions.
Applications
- Application Notes
- The FCGR2A antibody has been reported in applications of Blocking, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Enzyme-linked Immunosorbent Spot.
Target
- Alternative Names
- FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32), FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc recep
- Gene ID
- 2212
- UniProt ID
- P12318
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-983 | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3); Fab Fragment | FC | Mouse Fab |
| TAB-039WM-F(E) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); Fab Fragment | FC, WB, ELISA, Inhib | Human Fab |
| HPAB-0223-YC-F(E) | Mouse Anti-FCGR2A Recombinant Antibody Fab Fragment (HPAB-0223-YC-F(E)) | ELISA, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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