Mouse Anti-NHP FCGR2A Recombinant Antibody (clone AT10)
CAT#: VS-1024-XY91
This mouse-derived monoclonal antibody is finely tuned to target FCGR2A and exhibits reactivity with dog, human, non-human primate, and pig. The antibody has been validated for immunohistochemistry (IHC), flow cytometry (FC), and immunoprecipitation (IP) applications, making it a versatile reagent for comprehensive studies of FCGR2A across diverse species. Its ability to effectively perform in multiple assay systems highlights its suitability for in-depth research and diagnostic tasks.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1926_F1_3_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14730/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1847_F1_2_blue_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10718/26353_B_4_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL, HMC-1, K-562, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143226-FCGR2A
❮
❯
Specifications
- Immunogen
- K562 cell line
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- FCGR2A
- Species Reactivity
- Dog, Human, Non-human primate, Pig
- Clone
- AT10
- Applications
- IHC, FC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 1:500-1:800, FC: 20 µg/mL, IP: 20 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone AT10"
- CAT
- Product Name
See other products for "FCGR2A"
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-039WM-S(P) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); scFv Fragment | FC, WB, ELISA, Inhib | Human scFv |
| HPAB-0223-YC-S(P) | Mouse Anti-FCGR2A Recombinant Antibody scFv Fragment (HPAB-0223-YC-S(P)) | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-790CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone IV.3) | Block | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-791CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone AT10) | Neut, FC, IHC-Fr, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1272 | Hi-Affi™ Rabbit Anti-FCGR2A Recombinant Antibody (clone DS1272AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| FN-009CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3) | FC, Block | Mouse IgG1, κ |
| VS3-XY686 | Mouse Anti-FCGR2A Recombinant Antibody, FITC (clone 4AIV-3) | FC, IF | Mouse IgG2b |
| VS3-XY687 | Mouse Anti-FCGR2A Recombinant Antibody, PE (clone 4AIV-3) | FC, IF | Mouse IgG2b |
| VS3-XY688 | Mouse Anti-FCGR2A Recombinant Antibody (clone 4AIV-3) | FC, IF | Mouse IgG2b |
| VS3-FY540 | Recombinant Rabbit Anti-FCGR2A Antibody (clone R09-5D1) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY114 | CytoStream™ Mouse Anti-FCGR2A Recombinant Antibody (VS-0225-XY114) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY825 | Anti-FCGR2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY2480 | Anti-Human FCGR2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC341 | Recombinant Anti-FCGR2A Vesicular Antibody, EV Displayed (VS-0425-YC341) | ELISA, FC, Inhib, Cell-uptake |
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