Human Anti-Aβ Recombinant Antibody (clone h3D6) (CAT#: HPAB-1152WJ)

Figure 1 ScFv-h3D6-Pp purification.

SDS-PAGE of scFv-h3D6-Pp purification. (M) Molecular weight marker; (1) Supernatant after ammonium sulfate precipitation; (2) Pellet after ammonium sulfate precipitation; (3) Sample before Cationic Exchange chromatography (CEX); (4) CEX flow through; (5) CEX elution of the mean peak; (6) PBS-dialyzed scFv-h3D6-Pp.

Montoliu-Gaya, L., Esquerda-Canals, G., Bronsoms, S., & Villegas, S. (2017). Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect. PloS one, 12(8).

Figure 2 Therapeutic effects of scFv-hD6-Ec and scFv-h3D6-Pp.

Aβ42 ELISA of brain homogenates (Hippocampus and Cortex); Black: scFvh3D6-Ec, Red: scFv-h3D6-Pp, Grey: 3xTg-AD-Vehicle, Stripped: NTg-vehicle.

Montoliu-Gaya, L., Esquerda-Canals, G., Bronsoms, S., & Villegas, S. (2017). Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect. PloS one, 12(8).

Figure 3 Plasma concentrations of scFv-h3D6-WT and scFv-h3D6-EL.

Plasma concentrations of scFv-h3D6-WT and scFv-h3D6-EL were measured by competitive ELISA at 0h (for vehicle-injected mice), 1h, 5h, 10h, 1 day, 2 days, 5 days, and 8 days p.i.

Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).

Figure 4 ScFv-h3D6 influx into the brain. ScFv-h3D6 was detected and quantified by ELISA.

Comparative profiles of scFv-h3D6 in the plasma (dashed line) and nervous tissue (grey area). The inset within the plot magnifies late p.i. times (25-192h). The concentrations are expressed as microgram of scFv-h3D6 per milliliter of the plasma or brain protein extract and are represented as means and SEM.

Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).

Figure 5 ScFv-h3D6 influx into the brain.

Representative illustration, corresponding to the amygdalar region showing scFv-h3D6 in a 10-μm-thick brain section (at 5h p.i.).

Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).

Figure 6 Colocalization of scFv-h3D6 and Aβ peptide in the brain.

Colocalization of scFv-h3D6 and Aβ peptide in a 10-μm-thick brain section (at 5h p.i.). Scale bar, 100 μm.

Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).