Human Anti-Aβ Recombinant Antibody (clone h3D6)
CAT#: HPAB-1152WJ
This product is a humanized antibody h3D6 targeting human Aβ. This antibody can be used to treat, prevent, alleviate, reverse, or otherwise ameliorate symptoms or pathology or both, that are associated with Down's syndrome or pre-clinical or clinical Alzheimer's disease or cerebral amyloid angiopathy.
Published Data
PP
Figure 1 ScFv-h3D6-Pp purification.
SDS-PAGE of scFv-h3D6-Pp purification. (M) Molecular weight marker; (1) Supernatant after ammonium sulfate precipitation; (2) Pellet after ammonium sulfate precipitation; (3) Sample before Cationic Exchange chromatography (CEX); (4) CEX flow through; (5) CEX elution of the mean peak; (6) PBS-dialyzed scFv-h3D6-Pp.
Montoliu-Gaya, L., Esquerda-Canals, G., Bronsoms, S., & Villegas, S. (2017). Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect. PloS one, 12(8).
Inhib
Figure 2 Therapeutic effects of scFv-hD6-Ec and scFv-h3D6-Pp.
Aβ42 ELISA of brain homogenates (Hippocampus and Cortex); Black: scFvh3D6-Ec, Red: scFv-h3D6-Pp, Grey: 3xTg-AD-Vehicle, Stripped: NTg-vehicle.
Montoliu-Gaya, L., Esquerda-Canals, G., Bronsoms, S., & Villegas, S. (2017). Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect. PloS one, 12(8).
ELISA
Figure 3 Plasma concentrations of scFv-h3D6-WT and scFv-h3D6-EL.
Plasma concentrations of scFv-h3D6-WT and scFv-h3D6-EL were measured by competitive ELISA at 0h (for vehicle-injected mice), 1h, 5h, 10h, 1 day, 2 days, 5 days, and 8 days p.i.
Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).
ELISA
Figure 4 ScFv-h3D6 influx into the brain. ScFv-h3D6 was detected and quantified by ELISA.
Comparative profiles of scFv-h3D6 in the plasma (dashed line) and nervous tissue (grey area). The inset within the plot magnifies late p.i. times (25-192h). The concentrations are expressed as microgram of scFv-h3D6 per milliliter of the plasma or brain protein extract and are represented as means and SEM.
Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).
IF
Figure 5 ScFv-h3D6 influx into the brain.
Representative illustration, corresponding to the amygdalar region showing scFv-h3D6 in a 10-μm-thick brain section (at 5h p.i.).
Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).
IF
Figure 6 Colocalization of scFv-h3D6 and Aβ peptide in the brain.
Colocalization of scFv-h3D6 and Aβ peptide in a 10-μm-thick brain section (at 5h p.i.). Scale bar, 100 μm.
Esquerda-Canals, G., Martí-Clúa, J., & Villegas, S. (2019). Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment. PloS one, 14(5).
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human Aβ
- Species Reactivity
- Human
- Clone
- h3D6
- Applications
- PP, Inhib, ELISA, IF
- Related Disease
- Alzheimer's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Aβ antibody has been reported in applications of Protein Purification, Inhibition, Enzyme-linked Immunosorbent Assay, Immunofluorescence.
Target
- Alternative Names
- Amyloid beta; Aβ; Abeta
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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