Mouse Anti-ACOX1 Recombinant Antibody (VS3-FY2618)
CAT#: VS3-FY2618
This outstanding mouse recombinant monoclonal antibody demonstrates a high level of efficacy in its binding to ACOX1. The calculated molecular weight of the antigen is 74.4 kDa. It exhibits cross-reactivity with human. The antibody can be utilized for detection of ACOX1 by Western Blot, Immunohistochemistry, Immunocytochemistry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & peroxisomes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21195/395_B1_1_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21195/49200_A_5_6.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21195/49200_B_9_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21195/49200_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21195/49200_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161533-ACOX1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Acyl-CoA oxidase 1 of human
- Species Reactivity
- Human
- Applications
- WB, IHC, ICC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 74.4 kDa.
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/10-1/50
Target
- Alternative Names
- Acyl-CoA Oxidase 1; Palmitoyl-CoA Oxidase; Acyl-Coenzyme A Oxidase 1, Palmitoyl; Straight-Chain Acyl-CoA Oxidase; Acyl-CoA Oxidase 1, Palmitoyl; EC 1.3.3.6; ACOX
- Gene ID
- 51
- UniProt ID
- Q15067
- Sequence Similarities
- Belongs to the acyl-CoA oxidase family.
- Cellular Localization
- Peroxisome
- Post Translation Modifications
- Modification sites at PhosphoSitePlus
Modification sites at neXtProt
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047292138.1; XP_047292139.1; XP_054172311.1; XP_054172312.1; NP_001171968.1
- Function
- Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids (PubMed:7876265, 15060085, 17458872, 17603022, 32169171, 33234382).
Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ACOX1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0014MZ | Chicken Anti-ACOX1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3946 | Hi-Affi™ Rabbit Anti-ACOX1 Recombinant Antibody (clone SI6DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY18 | Recombinant Rabbit Anti-Acox1 Antibody (clone R08-2E5) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY85 | Anti-ACOX1 Immunohistochemistry Kit | IHC |
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