Mouse Anti-ACTA2 Recombinant Antibody (VS3-CJ1092)
CAT#: VS3-CJ1092
This product is a mouse antibody that recognizes human, mouse, and rat endogenous levels of Smooth Muscle Actin protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3761/630_C6_2_selected.jpg
Normal Tissue
Figure 2 Smooth muscle
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2/ihc_selected.jpg
Normal Tissue
Figure 3 Breast
Myoepithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_1_4.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166, HHSteC, HSkMC, hTERT-RPE1, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107796-ACTA2
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human Smooth Muscle Actin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat endogenous levels of Smooth Muscle Actin protein
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:10000-1:100000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:1000-1:2000).
Target
- Alternative Names
- ACTSA
- Gene ID
- 59
- UniProt ID
- P62736
- Sequence Similarities
- Belongs to the actin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-75 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
- Protein Refseq
- NP_001135417.1; NP_001307784.1; NP_001604.1
- Function
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACTA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0048 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone DS48AB) | IHC, ICC, FC | Rabbit IgG |
| MOR-4737 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone TH251DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0182C | Mouse Anti-ACTA2 Recombinant Antibody (clone 1E12) | WB, IHC-P, IF | Mouse IgG |
| ZG-0183C | Mouse Anti-ACTA2 Recombinant Antibody (clone 6A12) | WB, IF, IHC-P | Mouse IgG |
| VS3-QX11 | Mouse Anti-ACTA2 Recombinant Antibody (clone 4F4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX12 | Mouse Anti-ACTA2 Recombinant Antibody (clone 3F11-C2-G6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX13 | Mouse Anti-ACTA2 Recombinant Antibody (clone 7F12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY109 | Anti-ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY110 | Anti-Mouse ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY111 | Anti-Rabbit ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY112 | Anti-Rat ACTA2 Immunohistochemistry Kit | IHC |
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