Mouse Anti-ADAM7 Recombinant Antibody (clone 4D1)
CAT#: MRO-0685-CN
This antibody is a mouse monoclonal antibody that is capable of binding to ADAM7.
Gene Expression
Normal Tissue
Figure 1 High expression in epididymis
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8879/159867_A_3_6_selected.jpg
Normal Tissue
Figure 2 Low expression in stomach 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8879/159867_A_6_1_val_selected.jpg
Normal Tissue
Figure 3 Epididymis
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8879/159867_A_3_6.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (BEWO, NB-4, RPMI-8226, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000069206-ADAM7
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 4D1
- Applications
- WB, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Clear IHC Results
We employed this mouse monoclonal antibody for immunohistochemistry on rat tissue sections. Clone 4D1 demonstrated specific binding to ADAM7, helping us visualize the protein's localization. The Protein G affinity purification really seems to help with the signal-to-noise ratio in our imaging experiments.
Consistent Western Blotting
This recombinant antibody was used for Western blotting to detect ADAM7 in human cell lysates. The antibody is very stable and specific, reacting well across the species we tested, including mouse. The liquid format with glycerol made storage and handling very convenient for our team.
Q&As
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What species does this antibody react with and what are the applications?
A: This mouse monoclonal antibody (clone 4D1) is capable of binding to ADAM7 from Human, Mouse, and Rat samples. It has been validated for use in Western Blot (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC).
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How is this antibody purified and supplied?
A: The antibody is purified using Protein G affinity chromatography, achieving a purity of greater than 95% by SDS-PAGE. It is supplied as a liquid in 1*TBS (pH 7.4), 0.5% BSA, 50% Glycerol, and 0.05% Sodium Azide.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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