Rabbit Anti-ALDH7A1 Recombinant Antibody (clone R01-9D6) (CAT#: VS3-FY1643)
Clone R01-9D6 is a rabbit recombinant monoclonal antibody that specifically targets human ALDH7A1. ALDH7A protein has a calculated weight of 58 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R01-9D6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:20).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23296/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23296/if_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_B_7_5.jpg
Figure 3 Colon
(Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_8_3.jpg
(Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_8_4.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_4_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_4_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53675/169819_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164904-ALDH7A1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164904-ALDH7A1
Specifications
- Immunogen
- Synthetic peptide of human ALDH7A1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ALDH7A1
- Species Reactivity
- Human, Mouse
- Clone
- R01-9D6
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 58 kDa.
- Related Disease
- Epilepsy, Early-Onset, 4, Vitamin B6-Dependent and Hydrocephalus, Congenital, 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Aldehyde Dehydrogenase 7 Family Member A1; Alpha-AASA Dehydrogenase; P6c Dehydrogenase; Delta1-Piperideine-6-Carboxylate Dehydrogenase; Alpha-Aminoadipic Semialdehyde Dehydrogenase; Betaine Aldehyde Dehydrogenase; 26g Turgor Protein Homolog; Antiquitin-1; ATQ1; Delta1-Piperideine-6-Carboxylate Dehydrogenease
- Gene ID
- 501
- UniProt ID
- P49419
- Sequence Similarities
- Belongs to the aldehyde dehydrogenase family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Nucleus
- Post Translation Modifications
- Acetylation
- Protein Refseq
- NP_001173.2; NP_001188306.1; NP_001189333.2
- Function
- Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0143 | Hi-Affi™ Rabbit Anti-ALDH7A1 Recombinant Antibody (clone DS143AB) | WB, IHC | Rabbit IgG |
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