Rabbit Anti-ALPP Recombinant Antibody (clone P16-D)
CAT#: ZG-0351U
Rabbit anti-ALPP antibody (clone P16-D) displayed high specificity against the ALPP protein of Human.
Tested Data
Gene Expression
IHC-P
Figure 1 Rabbit Anti-ALPP Antibody (ZG-0351U) in IHC-P
Diffuse cytoplasmic PLAP positivity in the typical seminoma. Formalin fixed, paraffin embedded human tissue (4 μm section) stained with anti-PLAP monoclonal antibody.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38764/1046_F10_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38765/98876_A_1_7_selected.jpg
Normal Tissue
Figure 3 Placenta
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38764/98884_A_1_7.jpg
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Specifications
- Immunogen
- Peptide derived from the middle portion of human PLAP sequence
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ALPP
- Species Reactivity
- Human
- Clone
- P16-D
- Applications
- IHC-P
- Conjugate
- Unconjugated
- Related Disease
- Hypophosphatasia and Obstructive Jaundice.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 20 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Paraffin. Specific conditions should be optimized by the end user.
IHC-P: 1:100-1:200
Target
- Alternative Names
- ALP; IAP; ALPI; PALP; PLAP; PLAP-1; alkaline phosphatase, placental type; Intestinal alkaline phosphatase; Intestinal-type alkaline phosphatase; alkaline phosphatase Regan isozyme; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase 1; EC 3.1.3.1
- Gene ID
- 250
- UniProt ID
- P05187
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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