Rabbit Anti-ANXA5 Recombinant Antibody (clone R08-3A4)

CAT#: VS3-FY1633

The R08-3A4 recombinant monoclonal antibody has been developed to detect human ANXA5. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF. The antibody has the positive detection of annexin V by western blot analysis of annexin V in K562, C6, 3T3, Hela lysates.

Gene Expression Tested Data
Figure 1 IF staining of human cell line PC-3 Figure 2 IHC staining of human testis Figure 3 IHC staining of human placenta Figure 4 Pancreas Figure 5 Kidney Figure 6 Testis Figure 7 Lymph node Figure 8 RNA cell line category: Low cell line specificity
Figure 1 Anti-ANXA5 Antibody (VS3-FY1633) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human annexin V
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ANXA5
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R08-3A4
  • MW
  • The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
  • Related Disease
  • Pregnancy Loss, Recurrent 3 and Antiphospholipid Syndrome

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Annexin A5; Placental Anticoagulant Protein 4; Placental Anticoagulant Protein I; Vascular Anticoagulant-Alpha; Thromboplastin Inhibitor; Calphobindin I; Anchorin CII; Endonexin II; Lipocortin V; Annexin V; Annexin-5
  • Sequence Similarities
  • Belongs to the annexin family.
  • Cellular Localization
  • Collagen-containing extracellular matrix, cytoplasm, cytosol, endothelial microparticle, external side of plasma membrane, extracellular exosome, extracellular region, focal adhesion
  • Post Translation Modifications
  • S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Function
  • This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

See other products for "ANXA5"

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MOR-0186 Hi-Affi™ Rabbit Anti-ANXA5 Recombinant Antibody (clone DS186AB) ICC, IHC-P, WB Rabbit IgG
CAT Product Name Application Type
VS-0724-YC1323 AbPlus™ Anti-ANXA5 Magnetic Beads (VS-0724-YC1323) IP, Protein Purification
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