Rabbit Anti-AR Recombinant Antibody (VS13-YC62)
CAT#: VS13-YC62
The Rabbit Anti-AR Recombinant Antibody is specific for human AR. AR encodes the androgen receptor, a protein with functional domains for DNA binding and hormone interaction, implicated in diseases like spinal bulbar muscular atrophy due to polyglutamine tract expansions, with alternative splicing variants. The anti-AR antibody can be used for Western blot (1/500-1/1000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4733/1914_G6_27_cr5c8779fe8d0bf_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_6_6.jpg
Normal Tissue
Figure 3 High expression in seminal vesicle.
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_9_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, BJ hTERT+, HAP1, LHCN-M2, RH-30, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169083-AR
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Specifications
- Immunogen
- Recombinant human androgen receptor isform 3, aa 595-644.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AR
- Species Reactivity
- Human
- Applications
- WB, ICC, IF
- Conjugate
- NONE
- Related Disease
- Kennedy's disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- Androgen Receptor; Dihydrotestosterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 4; NR3C4; DHTR; Spinal And Bulbar Muscular Atrophy; Testicular Feminization; Kennedy Disease; HUMARA;
- Gene ID
- 367
- UniProt ID
- P10275
- Long Name
- Androgen Receptor
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_000035.2; NP_001011645.1; NP_001334990.1
- Function
- Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849).
Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024).
Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3:
Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Isoform 4:
Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0226 | Hi-Affi™ Rabbit Anti-AR Recombinant Antibody (clone DS226AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0307F | Mouse Anti-AR Recombinant Antibody (ZG-0307F) | WB, ELISA | Mouse IgG |
| ZG-0548J | Rabbit Anti-AR Recombinant Antibody (clone 5G7) | ELISA, WB, IHC | Rabbit IgG |
| ZG-0549J | Rabbit Anti-AR Recombinant Antibody (clone 10A5) | ELISA, IHC | Rabbit IgG |
| VS-0923-FY11 | Recombinant Mouse Anti-AR Antibody (VS-0923-FY11) | ELISA, WB | Mouse IgG2b |
| VS-0923-FY12 | Recombinant Mouse Anti-AR Antibody (VS-0923-FY12) | ELISA, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY24 | Rat Anti-NHP AR Recombinant Antibody (clone AN1-15) | WB, IHC, IF | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY143 | Anti-AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY485 | Anti-Mouse AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY484 | Anti-Human AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY486 | Anti-Monkey AR Immunohistochemistry Kit | IHC |
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