AibGenesis™ Rabbit Anti-ARHGAP25 Recombinant Antibody (AibG-Rb-AR251)
CAT#: VS-0426-FY251
This ARHGAP25-specific Rabbit IgG was developed through our AI-powered antibody discovery platform. Validated for WB and IHC in multiple species, it offers researchers a precise tool to study Rho GTPase-activating proteins and their regulatory roles in leukocyte signaling and inflammatory responses.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61395/1419_G8_3_selected.jpg
Normal Tissue
Figure 2 Spleen
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70561_B_9_4_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70561_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, NB-4, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163219-ARHGAP25
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Specifications
- Immunogen
- Human
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ARHGAP25
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-AR251
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated, Biotin and FITC are available.
- Related Disease
- Allergic Contact Dermatitis
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% Protective protein
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1:1000-1:5000), Immunohistochemistry (1:100-1:200)
Target
- Alternative Names
- KAIA0053; HEL-S-308
- Gene ID
- 9938
- UniProt ID
- P42331
- Long Name
- Rho GTPase Activating Protein 25
- Protein Refseq
- XP_011531509.1; XP_011531511.1; XP_016860915.1; XP_011531512.1
- Function
- GTPase-activating protein (GAP); negatively regulates Rac and Rho signaling in hematopoietic cells.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ARHGAP25"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0240 | Hi-Affi™ Rabbit Anti-ARHGAP25 Recombinant Antibody (clone DS240AB) | WB, IP, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1654 | Rabbit Anti-ARHGAP25 Recombinant Antibody (clone R07-6G6) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY502 | Anti-ARHGAP25 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY503 | Anti-Mouse ARHGAP25 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS14-YC232 | AibGenesis™ Rabbit Anti-ARHGAP25 Recombinant Antibody (AibG-Rb-GA33) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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