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Rabbit Anti-ATF1 Recombinant Antibody (clone R03-2E3) (CAT#: VS3-XY3268)

This antibody is a recombinant rabbit antibody binding with ATF1 (Activating transcription factor 1), which mediates PKA-induced stimulation of CRE-reporter genes. It recognizes and binds to human ATF1. It has been detected using Western Blot, Immunocytochemistry, Immunofluorescence and Immunoprecipitation.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55069/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55406/if_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/if_selected.jpg
Figure 4 IHC staining of human kidney
Figure 4 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate nuclear positivity in renal tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55069/ihc_selected.jpg
Figure 5 IHC staining of human kidney
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong nuclear positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/ihc_selected.jpg
Figure 6 IF staining of human cell line U-251 MG
Figure 6 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/952_A11_2_red_green.jpg
Normal Tissue
Figure 7 Cerebral cortex
Figure 7 Cerebral cortex
(Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/36029_B_9_5.jpg
Figure 8 Colon
Figure 8 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/36029_A_9_3.jpg
Figure 9 Liver
Figure 9 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/36029_A_8_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/36029_A_7_5.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16222/36029_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000123268-ATF1

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human ATF1
  • Species Reactivity
  • Human
  • Clone
  • R03-2E3
  • Applications
  • WB, ICC, IF, IP
  • Related Disease
  • Melanoma Of Soft Tissue; Fibrous Histiocytoma

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IF: 1/50-1/200
    IP: 1/20

Target

  • Alternative Names
  • TREB36; EWS-ATF1; FUS/ATF-1
  • Long Name
  • Activating transcription factor 1
  • Cellular Localization
  • Nucleus
  • Post Translation Modifications
  • Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
  • Function
  • This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
DrMAB-225 Human Anti-ATF1 Recombinant Antibody (clone U66) IP, IF Human IgG1
MOB-2525z Mouse Anti-ATF1 Recombinant Antibody (clone 22H9) WB, ELISA, IHC Mouse IgG1, κ
MOB-0698MZ Mouse Anti-ATF1 Recombinant Antibody (clone 4F8) IHC-P, sELISA Mouse IgG1
MRO-0129-CN Rabbit Anti-ATF1 Recombinant Antibody (clone CBACN-046) WB, IF, IHC, FC Rabbit IgG
VS3-CJ735 Rabbit Anti-ATF1 Recombinant Antibody (VS3-CJ735) WB, ICC, IF, IHC, FC Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0273 Hi-Affi™ Rabbit Anti-ATF1 Recombinant Antibody (clone DS273AB) WB, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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