Mouse Anti-ATF4 Recombinant Antibody (clone 2A6F12)
CAT#: VS7-HM147
Mouse Anti-ATF4 Recombinant Antibody (clone 2A6F12) is an mouse IgG1 antibody that recognizes the Human ATF4 by WB, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/638_E2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/44829_B_9_5.jpg
Normal Tissue
Figure 4 Testis
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/44829_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/44829_A_2_7.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128272-ATF4
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Specifications
- Immunogen
- Purified recombinant human ATF4 (AA: 212-351) fragment expressed in E. Coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ATF4
- Species Reactivity
- Human
- Clone
- 2A6F12
- Applications
- WB, IHC
- Conjugate
- None
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity-chromatography
- Format
- Liquid
- Molecular Weight
- 38.6kDa
- Concentration
- Please refer to the tube label.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice pack
Target
- Alternative Names
- ATF4; activating transcription factor 4; CREB2; TXREB; CREB-2; TAXREB67; cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive ele
- Gene ID
- 468
- UniProt ID
- P18848
- Protein Refseq
- NP_068352.2
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "ATF4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-226 | Human Anti-ATF4 Recombinant Antibody (clone U67) | IP, IF, WB | Human IgG1 |
| MRO-0131-CN | Rabbit Anti-ATF4 Recombinant Antibody (clone CBACN-048) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0553J | Rabbit Anti-ATF4 Recombinant Antibody (clone 9E1) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
| VS3-CJ736 | Rabbit Anti-ATF4 Recombinant Antibody (VS3-CJ736) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY193 | Anti-ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY578 | Anti-Rat ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY579 | Anti-Mouse ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY577 | Anti-Human ATF4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC73 | CytoStream™ Rabbit Anti-ATF4 Recombinant Antibody (VS13-YC73) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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