Rabbit Anti-ATF4 Recombinant Antibody (clone 9E1)
CAT#: ZG-0553J
This product is a rabbit antibody that recognizes ATF4.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-ATF4 Antibody (ZG-0553J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: ATF4 antibody at 1.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 KDa
Observed band size: 50 KDa
IHC
Figure 2 Rabbit Anti-ATF4 Antibody (ZG-0553J) in IHC
IHC image of ZG-0553J diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-ATF4 Antibody (ZG-0553J) in IF
Immunofluorescence staining of HepG2 cells with ZG-0553J at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 4 Rabbit Anti-ATF4 Antibody (ZG-0553J) in IP
Immunoprecipitating ATF4 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0553J in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0553J (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
FC
Figure 5 Rabbit Anti-ATF4 Antibody (ZG-0553J) in FC
Overlay histogram showing Hela cells stained with ZG-0553J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/638_E2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/44829_B_9_5.jpg
Normal Tissue
Figure 4 Testis
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/44829_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/44829_A_2_7.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128272-ATF4
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Specifications
- Immunogen
- A synthesized peptide derived from human ATF4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF4
- Species Reactivity
- Human
- Clone
- 9E1
- Applications
- ELISA, WB, IHC, IF, FC, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- CREB2; TXREB; CREB-2; TAXREB67; cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element B67; tax-responsive enhancer element-binding protein 67
- Gene ID
- 468
- UniProt ID
- P18848
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| DrMAB-226 | Human Anti-ATF4 Recombinant Antibody (clone U67) | IP, IF, WB | Human IgG1 |
| MRO-0131-CN | Rabbit Anti-ATF4 Recombinant Antibody (clone CBACN-048) | WB, IF, IHC, IP, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY193 | Anti-ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY578 | Anti-Rat ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY579 | Anti-Mouse ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY577 | Anti-Human ATF4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS13-YC73 | CytoStream™ Rabbit Anti-ATF4 Recombinant Antibody (VS13-YC73) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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