CytoStream™ Rabbit Anti-ATF4 Recombinant Antibody (VS13-YC73)
CAT#: VS13-YC73
The CytoStream™ Rabbit Anti-ATF4 Recombinant Antibody is specific for human, mouse and rat ATF4. ATF4, a transcription factor binding to tax-responsive elements and involved in stress response pathways, shares characteristics with other DNA-binding proteins like AP-1 and CREBs, with alternative transcripts and pseudogenes described. The anti-ATF4 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11596/638_E2_1_selected.jpg
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Specifications
- Immunogen
- Recombinant human ATF4, aa 1-220.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ATF4
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Activating Transcription Factor 4; Tax-Responsive Enhancer Element-Binding Protein 67; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP-Responsive Element-Binding Protein 2; CAMP-Dependent Transcription Factor ATF-4; Tax-Responsive Enhancer Element B67; DNA-Binding Protein TAXREB67; TAXREB67;
- Gene ID
- 468
- UniProt ID
- P18848
- Long Name
- Activating Transcription Factor 4
- Cellular Localization
- Nucleus; Nucleus speckle; Cytoplasm; Centrosome; Cell membrane
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ATF4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-226 | Human Anti-ATF4 Recombinant Antibody (clone U67) | IP, IF, WB | Human IgG1 |
| MRO-0131-CN | Rabbit Anti-ATF4 Recombinant Antibody (clone CBACN-048) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0553J | Rabbit Anti-ATF4 Recombinant Antibody (clone 9E1) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
| VS3-CJ736 | Rabbit Anti-ATF4 Recombinant Antibody (VS3-CJ736) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-HM147 | Mouse Anti-ATF4 Recombinant Antibody (clone 2A6F12) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY193 | Anti-ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY578 | Anti-Rat ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY579 | Anti-Mouse ATF4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY577 | Anti-Human ATF4 Immunohistochemistry Kit | IHC |
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