Mouse Anti-ATG5 Recombinant Antibody (VS4-WK40)
CAT#: VS4-WK40
This product is a mouse monoclonal antibody that recognizes ATG5 and can be used in ELISA, WB, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42973/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_8.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_B_2_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000057663-ATG5
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Specifications
- Immunogen
- Recombinant Protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ATG5
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- The antibody was purified from mouse ascites by immunogen affinity chromatography.
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% glycerol, pH 7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at -20°C or -80°C. Avoid repeated freeze.
- Shipping
- Ice packs
Applications
- Application Notes
- Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ATG5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0656MZ | Mouse Anti-ATG5 Recombinant Antibody (clone CBLS-114) | WB | Mouse IgG1 |
| MRO-0137-CN | Rabbit Anti-ATG5 Recombinant Antibody (clone CBACN-051) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-XY101 | Mouse Anti-ATG5 Recombinant Antibody (clone 10H7D2) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-WK1145 | Mouse Anti-ATG5 Recombinant Antibody (VS3-WK1145) | WB | Mouse IgG1, κ |
| VS3-WK1146 | Rabbit Anti-ATG5 Recombinant Antibody (VS3-WK1146) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0058MZ | Chicken Anti-ATG5 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0283 | Hi-Affi™ Rabbit Anti-ATG5 Recombinant Antibody (clone DS283AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY23 | AbPlus™ Anti-ATG5 Magnetic Beads (3C7) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC76 | CytoStream™ Rabbit Anti-ATG5 Recombinant Antibody (VS13-YC76) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY591 | Anti-ATG5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY592 | Anti-Mouse ATG5 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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