Rabbit Anti-ATM Recombinant Antibody (clone 3G11) (CAT#: ZG-0558J)
This product is a rabbit antibody that recognizes ATM.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IHC
Figure 1 Rabbit Anti-ATM Antibody (ZG-0558J) in IHC
IHC image of ZG-0558J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 2 Rabbit Anti-ATM Antibody (ZG-0558J) in IHC
IHC image of ZG-0558J diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
FC
Figure 3 Rabbit Anti-ATM Antibody (ZG-0558J) in FC
Overlay histogram showing Hela cells stained with ZG-0558J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human ATM
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATM
- Species Reactivity
- Human
- Clone
- 3G11
- Applications
- ELISA, IHC, FC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
IHC:1:50-1:200, FC:1:20-1:200
Target
- Alternative Names
- AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; serine-protein kinase ATM; A-T mutated; AT mutated; TEL1, telomere maintenance 1, homolog; ataxia telangiectasia mutated
- Gene ID
- 472
- UniProt ID
- Q13315
Related Resources
Gastric Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ATM"
Select a product category from the dropdown menu below to view related products.
Please select product type
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0059MZ | Chicken Anti-ATM Polyclonal IgY | WB | Chicken antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0558J. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-2.png)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA
Application: Neut, ELISA, Inhib, ICC, WB
Application: ELISA, IHC, FC, IP, IF, BL
Application: ELISA, IHC, WB
Application: ELISA, IHC, IF, IP
Application: ELISA, Neut
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
