Rabbit Anti-ATM Recombinant Antibody (clone R07-5J6) (CAT#: VS3-XY3271)
This product is a recombinant rabbit antibody specific for ATM (ATM serine/threonine kinase), which involves in pexophagy by mediating phosphorylation of PEX5. It recognizes and binds to human ATM. It also has cross-reactivity against mouse and rat ATM. It has been tested using Western Blot, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong nuclear positivity in tubules and glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_5_selected.jpg
(Immunohistochemical staining of human kidney shows strong nuclear positivity in tubules and glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_5_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_B_8_5.jpg
Figure 3 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_8_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_4.jpg
Figure 5 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_2_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_2_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_5.jpg
Figure 7 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_8_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149311-ATM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149311-ATM
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ATM
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-5J6
- Applications
- WB, ICC, IF, IP, FC
- Related Disease
- Ataxia-Telangiectasia; Mantle Cell Lymphoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
- Gene ID
- 472
- UniProt ID
- Q13315
- Long Name
- ATM serine/threonine kinase
- Cellular Localization
- Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Nucleus, Peroxisome
- Post Translation Modifications
- Phosphorylated by NUAK1/ARK5.
Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981.
Acetylated in vitro by KAT5/TIP60.
Deacetylated by SIRT7 during the late stages of DNA damage response, promoting ATM dephosphorylation and subsequent deactivation.
- Protein Refseq
- XP_006718906.1; XP_005271619.2
- Function
- Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Gastric Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ATM"
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0059MZ | Chicken Anti-ATM Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0286 | Hi-Affi™ Rabbit Anti-ATM Recombinant Antibody (clone DS286AB) | IF, IHC-P, IP, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0317F | Mouse Anti-ATM Recombinant Antibody (ZG-0317F) | WB, ELISA | Mouse IgG |
| ZG-0041J | Mouse Anti-ATM Recombinant Antibody (clone 1D1) | IHC-P, IF | Mouse IgG |
| ZG-0042J | Mouse Anti-ATM Recombinant Antibody (clone 3D3) | IHC-P, IF | Mouse IgG |
| ZG-0043J | Mouse Anti-ATM Recombinant Antibody (clone 4G9) | IHC-P, IF | Mouse IgG |
| VS4-WK43 | Mouse Anti-ATM Recombinant Antibody (VS4-WK43) | ELISA, IHC | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-XY3271. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.