Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) (CAT#: ZG-0047J)
This product is a mouse antibody that recognizes BCL2.
Specific Inquiry
WB
Figure 1 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in WB
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
WB
Figure 2 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in WB
Western blot detection of Bcl-2 in human breast cancer cell line MCF-7(A), MDA-MB-231(B) and Cal51(C) using Bcl-2 mouse mAb (1:2000 diluted). Predicted band size: 26kDa.Observed band size:26kDa.Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
IHC
Figure 3 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer.1, Using Bcl-2 Mouse mAb diluted at 1:200 (4°C overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital.
IF
Figure 4 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in IF
Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Bcl-2 Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
IHC
Figure 5 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in IHC
IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.
Specifications
- Immunogen
- Synthetic Peptide of Bcl-2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human BCL2
- Species Reactivity
- Human, Mouse, Rat, chicken
- Clone
- 6B5
- Applications
- IF/ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- Bcl-2; PPP1R50; apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; protein phosphatase 1, regulatory subunit 50
- Gene ID
- 596
- UniProt ID
- P10415
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-103-F(E) | Recombinant Anti-dog BCL2 Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-103-F(E) | Recombinant Human Anti-dog BCL2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-103-S(P) | Recombinant Anti-dog BCL2 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-103-S(P) | Recombinant Human Anti-dog BCL2 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-053 | Recombinant Anti-human BCL2 VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
| HPAB-0681-YJ-VHH | Camelid Anti-BCL2 Recombinant Single Domain Antibody (CBB72YJ) | ELISA | Camelid VHH |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-041CQ | Mouse Anti-BCL2 Recombinant Antibody (clone CBL685) | Neut | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0342 | Hi-Affi™ Rabbit Anti-BCL2 Recombinant Antibody (clone DS342AB) | IHC-P | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0405 | PE-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0406 | APC-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0407 | FITC-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0408 | A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0409 | PE-A*02:01/Human BCL2 (WLSLKTLLSL) MHC Tetramer | FCM, IHC |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1020CQ | Human Anti-BCL2 Recombinant Antibody (clone bcl-2/124) | WB, IF, FC, Apop | Human IgG1 |
| FAMAB-1021CQ | Human Anti-BCL2 Recombinant Antibody (clone bcl-2/100) | IP, WB, IHC | Human IgG1 |
| ZG-0675F | Mouse Anti-BCL2 Recombinant Antibody (clone 10C4) | WB | Mouse IgG1 |
| ZG-0104U | Rabbit Anti-BCL2 Recombinant Antibody (clone 3F6) | ELISA, WB, IHC | Rabbit IgG |
| ZG-0421U | Rabbit Anti-BCL2 Recombinant Antibody (clone P21-P), FITC | FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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