Mouse Anti-BCL2 Recombinant Antibody (clone 6B5)
CAT#: ZG-0047J
This product is a mouse antibody that recognizes BCL2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in WB
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
WB
Figure 2 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in WB
Western blot detection of Bcl-2 in human breast cancer cell line MCF-7(A), MDA-MB-231(B) and Cal51(C) using Bcl-2 mouse mAb (1:2000 diluted). Predicted band size: 26kDa.Observed band size:26kDa.Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
IHC
Figure 3 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer.1, Using Bcl-2 Mouse mAb diluted at 1:200 (4°C overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital.
IF
Figure 4 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in IF
Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Bcl-2 Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
IHC
Figure 5 Mouse Anti-BCL2 Recombinant Antibody (clone 6B5) in IHC
IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.
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Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55295/1484_G3_6_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_B_3_5.jpg
Normal Tissue
Figure 3 Colon
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_8_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HL-60, Karpas-707, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171791-BCL2
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Specifications
- Immunogen
- Synthetic Peptide of Bcl-2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human BCL2
- Species Reactivity
- Human, Mouse, Rat, chicken
- Clone
- 6B5
- Applications
- IF, ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
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Related Signaling Pathways
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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