Human Anti-BCL2 Recombinant Antibody (clone bcl-2/124) (CAT#: FAMAB-1020CQ)
This product is a recombinant human antibody that recognizes BCL2. The antibody was purified by affinity chromatography. It can be used in IP, WB, IHC.
Specific Inquiry
WB
Figure 1 IRS-1 associate with Bcl-2.
Murine IRS-1 in pRcCMV was cotransfected with human Bcl-2/pUC-CAGGS in 293 cells. Then lysates were immunoprecipitated and immunoblotted with indicated antibodies. 1, transfected; 2, not transfected.
Ueno, H., Kondo, E., Yamamoto-Honda, R., Tobe, K., Nakamoto, T., Sasaki, K., ... & Kadowaki, T. (2000). Association of insulin receptor substrate proteins with Bcl-2 and their effects on its phosphorylation and antiapoptotic function. Molecular Biology of the Cell, 11(2), 735-746.
IF
Figure 2 Colocalization of IRS-1 and Bcl-2.
CHO-IR/IRS-1/Bcl-2 cells were treated without insulin (100 nM) at 37°C for 10 min. Then cells were fixed and incubated with anti-IRS-1 antibody (C-20) and anti-Bcl-2 antibody (clone 124), followed by FITC-conjugated antirabbit (for C-20) or Texas Red-conjugated anti-mouse (for anti-Bcl-2 antibody) secondary antibodies.
Ueno, H., Kondo, E., Yamamoto-Honda, R., Tobe, K., Nakamoto, T., Sasaki, K., ... & Kadowaki, T. (2000). Association of insulin receptor substrate proteins with Bcl-2 and their effects on its phosphorylation and antiapoptotic function. Molecular Biology of the Cell, 11(2), 735-746.
FC
Figure 3 IRS-1 and IRS-2 but not IRS-3 suppress apoptosis of Ba/F3 cells deprived of IL-3.
FCS used in this study contained 15 nM insulin quantified by IRMA. The FACS analysis was performed 48 h after IL-3 depletion.
Ueno, H., Kondo, E., Yamamoto-Honda, R., Tobe, K., Nakamoto, T., Sasaki, K., ... & Kadowaki, T. (2000). Association of insulin receptor substrate proteins with Bcl-2 and their effects on its phosphorylation and antiapoptotic function. Molecular Biology of the Cell, 11(2), 735-746.
Apop
Figure 4 IRS-1 and IRS-2 but not IRS-3 suppress apoptosis of Ba/F3 cells deprived of IL-3.
Stable transfectants of IRS-1, IRS-2, IRS-3, 4F-IRS-1, Bcl-2, and mock cells were deprived of IL-3 and cultured in RPMI 1640 medium containing 5% FCS. FCS used in this study contained 15 nM insulin quantified by IRMA.
Ueno, H., Kondo, E., Yamamoto-Honda, R., Tobe, K., Nakamoto, T., Sasaki, K., ... & Kadowaki, T. (2000). Association of insulin receptor substrate proteins with Bcl-2 and their effects on its phosphorylation and antiapoptotic function. Molecular Biology of the Cell, 11(2), 735-746.
Specifications
- Immunogen
- Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human BLC-2
- Species Reactivity
- Human
- Clone
- bcl-2/124
- Applications
- WB, IF, FC, Apop
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence, Western Blot, Apoptosis and Flow Cytometry.
Target
- Alternative Names
- BCL2, Apoptosis Regulator; Protein Phosphatase 1, Regulatory Subunit 50; B-Cell CLL/Lymphoma 2; Apoptosis Regulator Bcl-2; PPP1R50; Bcl-2
- Gene ID
- 596
- UniProt ID
- P10415
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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|---|---|---|---|
| MOB-103-F(E) | Recombinant Anti-dog BCL2 Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-103-F(E) | Recombinant Human Anti-dog BCL2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-103-S(P) | Recombinant Anti-dog BCL2 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-103-S(P) | Recombinant Human Anti-dog BCL2 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-053 | Recombinant Anti-human BCL2 VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
| HPAB-0681-YJ-VHH | Camelid Anti-BCL2 Recombinant Single Domain Antibody (HPAB-0681-YJ-VHH) | ELISA | Camelid VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-103 | Recombinant Human Anti-dog BCL2 Antibody | RIA, ELISA, FuncS | IgG |
| MRO-0158-CN | Mouse Anti-BCL2 Recombinant Antibody (clone 9F1) | WB, IHC | Mouse IgG |
| MRO-0159-CN | Mouse Anti-BCL2 Recombinant Antibody (clone F7-1-A1) | WB, IF, IHC | Mouse IgG2b |
| MRO-0160-CN | Rabbit Anti-BCL2 Recombinant Antibody (clone CBACN-062) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-0161-CN | Rabbit Anti-BCL2 Recombinant Antibody (clone JF104-8) | WB, IF, IHC, FC | Rabbit IgG |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-041CQ | Mouse Anti-BCL2 Recombinant Antibody (clone CBL685) | Neut | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0342 | Hi-Affi™ Rabbit Anti-BCL2 Recombinant Antibody (clone DS342AB) | IHC-P | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0405 | PE-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-YF324 | A*02:01/Human BCL-2 (YLNRHLHTWI) MHC Pentamer | FCM | |
| MHC-YF325 | A*02:01/Human BCL-2 (PLFDFSWLSL) MHC Pentamer | FCM | |
| MHC-YF326 | A*02:01/Human BCL-2 (WLSLKTLLSL) MHC Pentamer | FCM | |
| MHC-YF327 | A*02:01/Human BCL-2 (ALSPVPPVV) MHC Pentamer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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