Mouse Anti-BCL2 Recombinant Antibody (VS3-CJ108) (CAT#: VS3-CJ108)

• Immunogen
• Endogenous Bcl-2 proteins

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human, Mouse and Rat BCL2

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IHC

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at -20°C.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:200).

• Alternative Names
• Bcl-2; PPP1R50

• Gene ID
• 596

• UniProt ID
• P10415

• Sequence Similarities
• Belongs to the Bcl-2 family.

• Cellular Localization
• Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

• Post Translation Modifications
• Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354).
  Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354).
  In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354).
  Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophag (PubMed:10567572, PubMed:18570871).
  Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).
  Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
  Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974).
  Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138).
  Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630).

• Protein Refseq
• NP_000624.2; NP_000648.2

• Function
• Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370).
  Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354).
  Appears to function in a feedback loop system with caspases (PubMed:11368354).
  Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354).
  Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974).
  May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS