Rabbit Anti-Btla Recombinant Antibody (clone R09-4A1)
CAT#: VS3-FY1676
Clone R09-4A1 is a rabbit IgG recombinant monoclonal antibody that is designed to specifically target human Btla protein, a calculated weight of 34 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-4A1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunoprecipitation (dilution range 1:20).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47211/ihc_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62029/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62029/148776_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186265-BTLA
❮
❯
Specifications
- Immunogen
- Recombinant mouse CD272 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse Btla
- Species Reactivity
- Human, Mouse
- Clone
- R09-4A1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 55-80 kDa.
- Related Disease
- Rheumatoid Arthritis and Arthritis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- B And T Lymphocyte Associated; B- And T-Lymphocyte-Associated Protein; B- And T-Lymphocyte Attenuator; CD272 Antigen; BTLA1; CD272
- Gene ID
- 208154
- UniProt ID
- Q7TSA3
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.1 Publication
N-glycosylated.
- Protein Refseq
- XP_011510749.1; XP_016861237.1; XP_047303452.1; XP_054201326.1; XP_054201327.1
- Function
- Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2.
May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1676. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "BTLA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1309CL | Human Anti-BTLA Recombinant Antibody (TAB-1309CL) | Inhib | Human IgG |
| TAB-1310CL | Human Anti-BTLA Recombinant Antibody (TAB-1310CL) | Block | Human IgG |
| TAB-1311CL | Human Anti-BTLA Recombinant Antibody (TAB-1311CL) | ELISA | Human IgG1 |
| TAB-1309CL-F(E) | Human Anti-BTLA Recombinant Antibody; Fab Fragment (TAB-1309CL-F(E)) | ELISA | Human Fab |
| TAB-1310CL-F(E) | Human Anti-BTLA Recombinant Antibody; Fab Fragment (TAB-1310CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-074CQ | Mouse Anti-BTLA Recombinant Antibody (clone PJ196) | Block, FC | Mouse IgG1 |
| NEUT-075CQ | Hamster Anti-BTLA Recombinant Antibody (clone 6A6) | FC, IP, Block | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-076CQ | Mouse Anti-BTLA Recombinant Antibody (clone 6F7) | FC, FuncS, Neut | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1605LY-S(P) | Human Anti-BTLA Recombinant Antibody; scFv Fragment (HPAB-1605LY-S(P)) | ELISA, FC | Humanized scfv |
| HPAB-M0448-YC-S(P) | Mouse Anti-BTLA Recombinant Antibody; scFv Fragment (HPAB-M0448-YC-S(P)) | FC | Mouse scFv |
| HPAB-0194-WJ-S(P) | Mouse Anti-BTLA Recombinant Antibody; scFv Fragment (HPAB-0194-WJ-S(P)) | ELISA, FC, WB, Neut | Mouse scFv |
| HPAB-0195-WJ-S(P) | Mouse Anti-BTLA Recombinant Antibody; scFv Fragment (HPAB-0195-WJ-S(P)) | ELISA, FC, WB, Neut | Mouse scFv |
| HPAB-0196-WJ-S(P) | Mouse Anti-BTLA Recombinant Antibody; scFv Fragment (HPAB-0196-WJ-S(P)) | ELISA, FC, WB, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1605LY | Human Anti-BTLA Recombinant Antibody (HPAB-1605LY) | ELISA, FC | Humanized IgG |
| FN-025CQ | Rat Anti-BTLA Recombinant Antibody (FN-025CQ) | FC, ELISA, FuncS | Rat IgG1 |
| FN-035CQ | Mouse Anti-BTLA Recombinant Antibody (clone ANC6E9) | ELISA, FC, FuncS | Mouse IgG1, κ |
| FN-079CQ | Mouse Anti-BTLA Recombinant Antibody (clone 8F4) | FC | Mouse IgG1, κ |
| VS-0923-FY23 | Recombinant Mouse Anti-BTLA Antibody (VS-0923-FY23) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1605LY-F(E) | Human Anti-BTLA Recombinant Antibody; Fab Fragment (HPAB-1605LY-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-M0448-YC-F(E) | Mouse Anti-BTLA Recombinant Antibody; Fab Fragment (HPAB-M0448-YC-F(E)) | FC | Mouse Fab |
| HPAB-0194-WJ-F(E) | Mouse Anti-BTLA Recombinant Antibody; Fab Fragment (HPAB-0194-WJ-F(E)) | ELISA, FC, WB, Neut | Mouse Fab |
| HPAB-0195-WJ-F(E) | Mouse Anti-BTLA Recombinant Antibody; Fab Fragment (HPAB-0195-WJ-F(E)) | ELISA, FC, WB, Neut | Mouse Fab |
| HPAB-0196-WJ-F(E) | Mouse Anti-BTLA Recombinant Antibody; Fab Fragment (HPAB-0196-WJ-F(E)) | ELISA, FC, WB, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY6 | CytoStream™ Mouse Anti-BTLA Recombinant Antibody (VS-0225-XY6) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY62 | Human Anti-BTLA (clone icatolimab) scFv-Fc Chimera | ELISA, FC | Human IgG4, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC609 | Recombinant Anti-BTLA Vesicular Antibody, EV Displayed (VS-0425-YC609) | ELISA, FC, SPR, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY855 | Anti-BTLA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: WB, IHC, FC, Cyt, ELISA
Application: WB, ELISA, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
Application: WB, FC, IF, Inhib, ELISA, IHC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
