Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137)
CAT#: PABJ-0137
This product is a Camelid antibody that recognizes C. difficile toxin B. 7F inhibits GTD cleavage, but does not directly interact with the CPD. 7F binds to the C-terminus of the GTD, immediately juxtaposed to the cleavage site.
Tested Data
SDS-PAGE
Figure 1 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in SDS-PAGE
SDS-PAGE analysis of PABJ-0137 in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced protein migrates as about 14 kDa.
SEC-HPLC
Figure 2 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in SEC-HPLC
The purity of PABJ-0137 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in WB
Western blot analysis of PABJ-0137 was performed by loading Toxin B/TcdB-C. difficile (His).
PABJ-0137 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Rabbit anti-Camelid VHH antibody
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.8 μg)
ELISA
Figure 4 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in ELISA
ELISA analysis of PABJ-0137 was performed by coating with Toxin B/TcdB-C. difficile (His).
The secondary antibody: HRP-Rabbit anti-Camelid VHH antibody
DB
Figure 5 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in DB
Dot Blot analysis of PABJ-0137 was performed by coating with Toxin B/TcdB-C. difficile (His).
The secondary antibody: HRP-Rabbit anti-Camelid VHH antibody
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Specifications
- Host Species
- Camelid
- Derivation
- Camel
- Type
- Camelid VHH
- Specificity
- C. difficile toxin B
- Species Reactivity
- C. difficile
- Clone
- PABJ-0137
- Applications
- ELISA, Inhib
- Related Disease
- C. difficile infection (CDI)
Product Property
- Purification
- Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Inhibition.
Target
- Alternative Names
- Clostridium difficile toxin B; C. difficile toxin B; C. difficile; Clostridium difficile; TcdB
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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