Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) (CAT#: PABJ-0137)
This product is a Camelid antibody that recognizes C. difficile toxin B. 7F inhibits GTD cleavage, but does not directly interact with the CPD. 7F binds to the C-terminus of the GTD, immediately juxtaposed to the cleavage site.
Specific Inquiry
SDS-PAGE
Figure 1 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in SDS-PAGE
SDS-PAGE analysis of PABJ-0137 in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced protein migrates as about 14 kDa.
SEC-HPLC
Figure 2 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in SEC-HPLC
The purity of PABJ-0137 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in WB
Western blot analysis of PABJ-0137 was performed by loading Toxin B/TcdB-C. difficile (His).
PABJ-0137 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Rabbit anti-Camelid VHH antibody
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.8 μg)
ELISA
Figure 4 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in ELISA
ELISA analysis of PABJ-0137 was performed by coating with Toxin B/TcdB-C. difficile (His).
The secondary antibody: HRP-Rabbit anti-Camelid VHH antibody
DB
Figure 5 Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0137) in DB
Dot Blot analysis of PABJ-0137 was performed by coating with Toxin B/TcdB-C. difficile (His).
The secondary antibody: HRP-Rabbit anti-Camelid VHH antibody
Specifications
- Host Species
- Camelid
- Derivation
- Camel
- Type
- Camelid VHH
- Specificity
- C. difficile toxin B
- Species Reactivity
- C. difficile
- Clone
- PABJ-0137
- Applications
- ELISA, Inhib
- Related Disease
- C. difficile infection (CDI)
Product Property
- Purification
- Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Inhibition.
Target
- Alternative Names
- Clostridium difficile toxin B; C. difficile toxin B; C. difficile; Clostridium difficile; TcdB
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-274-sdAb | Recombinant Anti-C. diff toxin B VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| PABJ-0136 | Camelid Anti-C. difficile toxin B Recombinant Antibody (PABJ-0136) | ELISA, Neut | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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