Mouse Anti-C3AR1 Recombinant Antibody (clone 2A1) (CAT#: FAMAB-0111JF)
Recombinant mouse monoclonal antibody binds to the second extracellular loop of the human C3a receptor (2e loop). This anti-C3a antibody (2A1) can be useful in the study of immune processes of arthritis, diet-induced obesity and cancers.
Specific Inquiry
IHC
Figure 1 Spinal localization of C3aR1.
Scale bars: I, 100 μm; I1–4, 50 μm.
Matsumoto, N., Satyam, A., Geha, M., Lapchak, P. H., Dalle Lucca, J. J., Tsokos, M. G., & Tsokos, G. C. (2017). C3a enhances the formation of intestinal organoids through C3aR1. Frontiers in immunology, 8, 1046.
WB
Figure 2 Protein expression of Ki67 and Lgr5 were compared in organoids by western blotting in the absence or presence of C3a in the medium.
β-actin was used as a protein loading control (n = 3 mice in each group).
Matsumoto, N., Satyam, A., Geha, M., Lapchak, P. H., Dalle Lucca, J. J., Tsokos, M. G., & Tsokos, G. C. (2017). C3a enhances the formation of intestinal organoids through C3aR1. Frontiers in immunology, 8, 1046.
FuncS
Figure 3 C3a promotes organoid formation.
The number of organoids formed in wells 7 days were counted after the initiation of culture under various conditions.
Matsumoto, N., Satyam, A., Geha, M., Lapchak, P. H., Dalle Lucca, J. J., Tsokos, M. G., & Tsokos, G. C. (2017). C3a enhances the formation of intestinal organoids through C3aR1. Frontiers in immunology, 8, 1046.
Specifications
- Immunogen
- Second extracellular loop of the C3aR
- Host Species
- Mouse
- Derivation
- Human phage display scFv library
- Type
- Mouse IgG
- Specificity
- Human C3AR1
- Species Reactivity
- Human
- Clone
- 2A1
- Applications
- WB, IHC, FuncS
- Related Disease
- Arthritis, diet-induced obesity, cancers
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The C3AR1 antibody has been reported in applications of Western Blot, Immunohistochemistry, Functional Assay.
Target
- Alternative Names
- C3AR1; complement component 3a receptor 1; AZ3B; C3AR; HNFAG09; C3a receptor
- Gene ID
- 719
- UniProt ID
- Q16581
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1858z | Mouse Anti-C3AR1 Recombinant Antibody (clone 25H10) | ELISA, FC | Mouse IgG1, κ |
| MOB-0279CT | Mouse Anti-C3AR1 Recombinant Antibody (clone 85) | FC, IHC-Fr | Mouse IgG1 |
| FAMAB-0110JF | Mouse Anti-C3AR1 Recombinant Antibody (clone 3G7) | IHC, FC | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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