Rabbit Anti-CALM1 Recombinant Antibody (clone CBACN-078)
CAT#: MRO-0213-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to CALM1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18558/924_C6_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18558/40729_B_9_8_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44999/102088_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44999/102088_A_5_6.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44999/102088_A_8_3.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44999/102088_B_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198668-CALM1
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Specifications
- Immunogen
- Full length protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse, Rat
- Clone
- CBACN-078
- Applications
- WB, IF, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Detects CALM1 In Multiple Species
One of the best features of this rabbit monoclonal antibody is its reactivity with Human, Mouse, and Rat samples. We used it for Western blotting and got clear bands at the expected molecular weight across all three species. A very versatile antibody.
High Quality IF Images
We utilized clone CBACN-078 for immunofluorescence staining of CALM1. The images were sharp with low background, allowing us to precisely localize the protein within the cells. It also worked well for immunoprecipitation, pulling down the target efficiently.
Q&As
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Is this a rabbit monoclonal antibody?
A: Yes, this is a recombinant rabbit monoclonal antibody (clone CBACN-078). It is produced using recombinant technology and purified via Protein A affinity to ensure high specificity and consistency for your research.
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Does this antibody react with mouse samples?
A: Yes, this antibody is capable of binding to CALM1 from Human, Mouse, and Rat species. It has been validated for use in Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunoprecipitation (IP).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CALM1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0441 | Hi-Affi™ Rabbit Anti-CALM1 Recombinant Antibody (clone DS441AB) | WB, FC, IP, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-053CQ | Mouse Anti-CALM1 Recombinant Antibody (clone CBLS-191) | WB, ELISA, FC, IF, IHC, IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ1086 | Rabbit Anti-CALM1 Recombinant Antibody (VS3-CJ1086) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY940 | Anti-CALM1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY941 | Anti-Mouse CALM1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY942 | Anti-Rat CALM1 Immunohistochemistry Kit | IHC |
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