Mouse Anti-CAPN2 Recombinant Antibody (VS3-FY2643)
CAT#: VS3-FY2643
The anti-CAPN2 antibody is a mouse recombinant monoclonal antibody, which has been intricately crafted to target the CAPN2. The calculated molecular weight of the antigen is 80.0 kDa. It cross-reacts with human and mouse. This monoclonal antibody is potential for conducting research on Alzheimer's disease. This monoclonal antibody can be used in Western Blot, Immunohistochemistry, Flow cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24470/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate positivity in subsets of urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24470/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24470/53623_B_7_5.jpg
Normal Tissue
Figure 4 Bronchus
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24470/53623_B_5_2.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24470/53623_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162909-CAPN2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Calpain 2 of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 80.0 kDa.
- Related Disease
- Alzheimer's disease
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/100
Target
- Alternative Names
- Calpain 2; Calcium-Activated Neutral Proteinase 2; Calpain 2, (M/II) Large Subunit; Millimolar-Calpain; Calpain M-Type; EC 3.4.22.53; M-Calpain; CANP 2; CANPL2; Calpain 2, Large [Catalytic] Subunit
- Gene ID
- 824
- UniProt ID
- P17655
- Sequence Similarities
- Belongs to the peptidase C2 family.
- Cellular Localization
- Cytoplasm
Cell membrane
- Post Translation Modifications
- Ubiquitination at Lys26, Lys260, Lys476, and Lys674
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047287300.1; XP_054194910.1; NP_001139540.1; NP_001739.3
- Function
- Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Proteolytically cleaves MYOC at 'Arg-226'
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CAPN2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2088z | Mouse Anti-CAPN2 Recombinant Antibody (clone 3B9) | ELISA, WB | Mouse IgG1 |
| MRO-0217-CN | Rabbit Anti-CAPN2 Recombinant Antibody (clone JA43-41) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0702F | Mouse Anti-CAPN2 Recombinant Antibody (clone 28F3) | IP | Mouse IgG1 |
| VS3-CJ717 | Mouse Anti-CAPN2 Recombinant Antibody (VS3-CJ717) | WB, IHC-P, FC | Mouse IgG2b |
| VS4-WK59 | Rabbit Anti-CAPN2 Recombinant Antibody (clone 17E9) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0461 | Hi-Affi™ Rabbit Anti-CAPN2 Recombinant Antibody (clone DS461AB) | FC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY308 | Anti-CAPN2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY973 | Anti-Human CAPN2 Immunohistochemistry Kit | IHC |
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