CytoStream™ Rabbit Anti-Casp1 Polyclonal Antibody (VS13-YC123) (CAT#: VS13-YC123)

• Immunogen
• Recombinant mouse Caspase-1 p20 (aa 181-280) conjugated with KLH.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat Casp1

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF

• Conjugate
• NONE

• Clonality
• Polyclonal

• Purification
• Affinity Chromatography purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4)

• Preservative
• 15 mM Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/2000
  ELISA: 1/5000-1/10000
  Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
  Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
  Flow cytometry (FC): 1 μg per 1x 10⁶ cells
  Immunocytochemistry (ICC): 1/100-1/500
  Immunofluorescence (IF): 1/100-1/500

• Alternative Names
• ICE; Il1bc

• Gene ID
• 12362

• UniProt ID
• P29452

• Long Name
• caspase 1

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cell membrane; Cytoplasm

• Protein Refseq
• NP_001214.1; NP_001244047.1; NP_001244048.1

• Function
• Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255).
  Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255).
  Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465).
  Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003).
  In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275).
  Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:22464733).
  Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
  In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).
  Isoform Delta: Apoptosis inactive.
  Isoform Epsilon: Apoptosis inactive.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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