CytoStream™ Rabbit Anti-Casp1 Polyclonal Antibody (VS13-YC123)
CAT#: VS13-YC123
The CytoStream™ Rabbit Anti-Casp1 Polyclonal Antibody is specific for human, mouse and rat Casp1. Casp1, a cysteine endopeptidase, plays roles in apoptosis, inflammation, and cytokine production, located in various tissues, part of the IPAF inflammasome complex. The anti-Casp1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2685/634_E8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong nuclear and cytoplasmic positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3056/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2685/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2685/639_E8_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2685/635_E8_3_blue_red_green.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2685/6753_A_7_3.jpg
Normal Tissue
Figure 7 Testis
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2685/6753_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+, HBEC3-KT, HHSteC, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137752-CASP1
❮
❯
Specifications
- Immunogen
- Recombinant mouse Caspase-1 p20 (aa 181-280) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat Casp1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4)
- Preservative
- 15 mM Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- ICE; Il1bc
- Gene ID
- 12362
- UniProt ID
- P29452
- Long Name
- caspase 1
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cell membrane; Cytoplasm
- Protein Refseq
- NP_001214.1; NP_001244047.1; NP_001244048.1
- Function
- Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255).
Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255).
Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465).
Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003).
In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275).
Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:22464733).
Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).
Isoform Delta: Apoptosis inactive.
Isoform Epsilon: Apoptosis inactive.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS13-YC123. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
NLR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Casp1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-209-sdAb | Recombinant Anti-Mouse Casp1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3801z | Mouse Anti-CASP1 Recombinant Antibody (clone 12G12) | WB, IF, IHC, IP | Mouse IgG1, κ |
| MOB-0323CT | Mouse Anti-CASP1 Recombinant Antibody (clone EML275) | IHC-P, WB | Mouse IgG2a |
| MRO-1718-CN | Rabbit Anti-CASP1 Polyclonal Antibody (MRO-1718-CN) | WB, IHC | Rabbit IgG |
| MRO-1719-CN | Rabbit Anti-CASP1 Polyclonal Antibody (MRO-1719-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-FY190 | Recombinant Rabbit Anti-CASP1 Antibody (clone R05-7H1) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0471 | Hi-Affi™ Rabbit Anti-CASP1 Recombinant Antibody (clone DS471AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY993 | Anti-CASP1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, WB, BLI, SPR
Application: Block, Cyt, FuncS, Inhib
Application: FuncS, Inhib, IP, ELISA
Application: WB, ELISA, FC, IHC, IP
Application: WB, IHC, ELISA, FC
Application: Neut, ELISA, WB, SPR, IF, EM
-2.png)
Application: ELISA
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
