Rabbit Anti-CASP8 Recombinant Antibody (VS3-WK1583)
CAT#: VS3-WK1583
This antibody is a rabbit recombinant antibody against human CASP8, which is involved in the programmed cell death induced by Fas and various apoptotic stimuli. This clone can be used in WB analysis at a dilution of 1/500-2000 and in ELISA analysis at a dilution of 1/5000-20000.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1302/69_H9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6191/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2047/5614_A_8_2_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1302/30722_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1302/30722_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1302/30722_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000064012-CASP8
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Specifications
- Immunogen
- Synthesized peptide derived from human Caspase 8 (p18, Cleaved-Asp374)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CASP8
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/2000
ELISA: 1/5000-1/20000
Target
- Alternative Names
- Caspase 8; Caspase 8, Apoptosis-Related Cysteine Peptidase; Caspase 8, Apoptosis-Related Cysteine Protease; MORT1-Associated Ced-3 Homolog; ICE-Like Apoptotic Protease 5; Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; FADD-Like ICE; Casp-8; FLICE; CAP4
- Gene ID
- 841
- UniProt ID
- Q14790
- Long Name
- Caspase 8
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cell projection, Cytoplasm, Nucleus
- Post Translation Modifications
- Generation of the p10 and p18 subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
- Protein Refseq
- NP_001073593.1
- Function
- Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "CASP8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1464z | Mouse Anti-CASP8 Recombinant Antibody (clone 24C10) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0330CT | Mouse Anti-CASP8 Recombinant Antibody (clone 1H11) | WB | Mouse IgG1 |
| MRO-0244-CN | Rabbit Anti-CASP8 Recombinant Antibody (clone CBACN-093) | WB, IF, IHC | Rabbit IgG |
| MRO-0245-CN | Rabbit Anti-CASP8 Recombinant Antibody (clone CBACN-094) | WB, IF, IHC | Rabbit IgG |
| ZG-0072J | Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) | WB, IF, ICC, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0082MZ | Chicken Anti-Caspase 8 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0479 | Hi-Affi™ Rabbit Anti-CASP8 Recombinant Antibody (clone DS479AB) | FC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY321 | Anti-CASP8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1009 | Anti-Mouse CASP8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1012 | Anti-Human CASP8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1010 | Anti-Monkey CASP8 Immunohistochemistry Kit | IHC |
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