Mouse Anti-CCNB1 Recombinant Antibody (clone 1A5)
CAT#: ZG-0205J
This product is a mouse antibody that recognizes CCNB1.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-CCNB1 Recombinant Antibody (clone 1A5) in IHC
Immunohistochemical analysis of paraffin-embedded Human lung Tissue using Cyclin B1 Mouse mAb diluted at 1:200.
IHC
Figure 2 Mouse Anti-CCNB1 Recombinant Antibody (clone 1A5) in IHC
Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Cyclin B1 Mouse mAb diluted at 1:200.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
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Specifications
- Immunogen
- Synthetic Peptide of Cyclin B1 at AA range of 60-140
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CCNB1
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 1A5
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CCNB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1647z | Mouse Anti-CCNB1 Recombinant Antibody (clone 28C1) | WB, IF, IHC, IP, FuncS | Mouse IgG1, κ |
| MRO-0410-CN | Mouse Anti-CCNB1 Recombinant Antibody (clone C2-F2) | WB, IF | Mouse IgG1 |
| MRO-0411-CN | Rabbit Anti-CCNB1 Recombinant Antibody (clone CBACN-162) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-CJ654 | Rabbit Anti-CCNB1 Recombinant Antibody (VS3-CJ654) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY180 | Mouse Anti-CCNB1 Recombinant Antibody (clone 1B10) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0514 | Hi-Affi™ Rabbit Anti-CCNB1 Recombinant Antibody (clone DS514AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4322 | PE-B*57:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4323 | PE-A*29:02/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4324 | PE-B*58:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4410 | PE-A*01:01/Human CCNB1 (CSEYVKDIY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY146 | Mouse Anti-NHP CCNB1 Recombinant Antibody (clone V152) | WB, IHC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY334 | Anti-CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1081 | Anti-Mouse CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1080 | Anti-Human CCNB1 Immunohistochemistry Kit | IHC |
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