Rabbit Anti-CCNE1 (pT77) Recombinant Antibody (clone R02-6U7)
CAT#: VS3-WK2145
This product is a rabbit recombinant antibody that is specific to phosphorylated CCNE1. The target of this antibody is phospho-CCNE1 (Thr77). The clone R02-6U7 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, and IHC staining at a dilution of 1/50-1/100. The molecular weight of CCNE1 (pThr77) observed in WB experiments using this antibody is 47 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr77 of human CCNE1
- Species Reactivity
- Human
- Clone
- R02-6U7
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-WK2145. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "CCNE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B078(A) | Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B078(G) | Recombinant Anti-human CCNE1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-Tat |
| IAB-B078(T) | Recombinant Anti-human CCNE1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0517 | Hi-Affi™ Rabbit Anti-CCNE1 Recombinant Antibody (clone DS517AB) | FC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0415-CN | Rabbit Anti-CCNE1 Recombinant Antibody (clone CBACN-166) | WB, IF | Rabbit IgG |
| VS3-CJ810 | Rabbit Anti-CCNE1 Recombinant Antibody (VS3-CJ810) | WB, ICC, IF | Rabbit IgG |
| VS3-CJ811 | Rabbit Anti-CCNE1 (phospho T77) Recombinant Antibody (VS3-CJ811) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-FY220 | Recombinant Mouse Anti-CCNE1 Antibody (clone 1C8D4) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY221 | Recombinant Mouse Anti-CCNE1 Antibody (clone 6C10B2) | ELISA, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1090 | Anti-CCNE1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application:
Application: ELISA, IHC, FC, WB, IF, IA
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: FC
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
