Human Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-0884-WJ-F(E))
CAT#: HPAB-0884-WJ-F(E)
This is a humanized monoclonal antibody, HPAB-0884-WJ, that targets CD34, a surface antigen found on human stem cells. The binding affinity of HPAB-0884-WJ to KG1a cells was determined to be 6.9 nM, as measured by the KD value. HPAB-0884-WJ-F(E) antibody is the Fab fragment antibody form of HPAB-0884-WJ antibody. Fab fragments, the antigen-binding domains of an antibody molecule, consist of two amino acid chains composed of VH+CH1 and VL+CL domains.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36723/1751_G1_7_cr57fd220c70be0_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HUVEC TERT2, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174059-CD34
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized Fab
- Specificity
- Human CD34
- Species Reactivity
- Human
- Applications
- ELISA
- Related Disease
- Malignant hematologic diseases, Solid tumors.
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Reduced immune activation
The lack of Fc region in this Fab fragment has resulted in reduced immune activation in our in vivo studies. This characteristic has made our experiments more straightforward and reduced potential variables, allowing us to focus on the specific effects of CD34 targeting on stem cells.
Enhanced clarity in imaging
Using the Human Anti-CD34 Recombinant Antibody; Fab Fragment has enhanced the clarity of our imaging studies. The reduced non-specific binding and exceptional specificity for CD34 have allowed us to obtain more precise and interpretable results in our regenerative medicine research.
Valuable for vascular biology
This Fab fragment has proven invaluable in our vascular biology research. The specific targeting of CD34-positive endothelial progenitor cells has allowed us to delve deeper into angiogenesis and vascular repair mechanisms, greatly enhancing our understanding of these processes.
Q&As
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What distinguishes a human anti-CD34 recombinant antibody's Fab fragment from an antibody's full-length counterpart?
A: The Fab fragment consists only of the antigen-binding region of the antibody, which includes the variable regions of the heavy and light chains. It lacks the Fc region found in full-length antibodies, resulting in reduced immune activation and improved penetration into tissues, making it ideal for certain diagnostic and therapeutic applications.
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What benefits over conventional antibodies does the Human Anti-CD34 Recombinant Antibody; Fab Fragment offer?
A: The Fab fragment offers several advantages, including better tissue penetration due to its smaller size, reduced non-specific binding and lower background noise, and fewer immune activation effects, making it ideal for precise and sensitive detection in various assays.
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What role does the CD34 antigen play in biological research?
A: The CD34 antigen is a critical marker for identifying and isolating hematopoietic stem and progenitor cells, as well as endothelial progenitor cells. It is essential in stem cell biology, regenerative medicine, and hematological research for studying cell differentiation, migration, and therapeutic potential.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-175-F(E) | Recombinant Anti-human CD34 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| HPAB-1042-FY-S(P) | Human Anti-CD34 Recombinant Antibody (clone c4C8); Fab Fragment | ELISA | Chimeric (mouse/human) Fab |
| HPAB-1043-FY-S(P) | Human Anti-CD34 Recombinant Antibody (clone C5B12); Fab Fragment | ELISA | Chimeric (mouse/human) Fab |
| HPAB-1044-FY-S(P) | Human Anti-CD34 Recombinant Antibody (clone C2e10); Fab Fragment | ELISA | Chimeric (mouse/human) Fab |
| HPAB-0883-WJ-F(E) | Mouse Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-0883-WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-175-S(P) | Recombinant Human Anti-human CD34 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| HPAB-M0472-YC-S(P) | Mouse Anti-CD34 Recombinant Antibody; scFv Fragment (HPAB-M0472-YC-S(P)) | WB, IF, IHC, IP, WB | Mouse scFv |
| HPAB-1042-FY-F(E) | Mouse Anti-CD34 Recombinant Antibody (clone c4C8); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1043-FY-F(E) | Mouse Anti-CD34 Recombinant Antibody (clone C5B12); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1044-FY-F(E) | Mouse Anti-CD34 Recombinant Antibody (clone C2e10); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-149LC | Anti-Human CD34 Recombinant Antibody (h4C8) | FC, Inhib | Humanized antibody |
| TAB-149LC-S(P) | Anti-Human CD34 Recombinant Antibody scFv Fragment (h4C8) | ELISA | Humanized antibody |
| TAB-149LC-F(E) | Anti-Human CD34 Recombinant Antibody Fab Fragment (h4C8) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-150LC | Human Anti-CD34 Recombinant Antibody (TAB-150LC) | ELISA | Human IgG |
| TAB-150LC-S(P) | Human Anti-CD34 Recombinant Antibody; scFv Fragment (TAB-150LC-S(P)) | ELISA | Human scFv |
| TAB-150LC-F(E) | Human Anti-CD34 Recombinant Antibody; Fab Fragment (TAB-150LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0356MZ | Mouse Anti-CD34 Recombinant Antibody (clone CIRMA-K4) | ELISA | Mouse IgG1 |
| HPAB-M0472-YC | Mouse Anti-CD34 Recombinant Antibody (HPAB-M0472-YC) | WB, IF, IHC, IP, WB | Mouse IgG |
| HPAB-M0473-YC | Human Anti-CD34 Recombinant Antibody (HPAB-M0473-YC) | ELISA | Chimeric (mouse/human) IgG |
| HPAB-M0474-YC | Human Anti-CD34 Recombinant Antibody (HPAB-M0474-YC) | ELISA | Humanized IgG |
| ZG-0383F | Mouse Anti-CD34 Recombinant Antibody (ZG-0383F) | WB, IHC, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-342CQ | Mouse Anti-CD34 Recombinant Antibody (clone 4H11) | Block, FC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0554 | Hi-Affi™ Rabbit Anti-CD34 Recombinant Antibody (clone DS554AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC90 | AbPlus™ Anti-Cd34 Magnetic Beads (VS-0724-YC90) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC26 | Rabbit Anti-CD34 Antibody (VS-0924-YC26) - Cancer Stem Cell Marker | IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY92 | Mouse Anti-NHP CD34 Recombinant Antibody (clone 581) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY38 | CytoStream™ Mouse Anti-CD34 Recombinant Antibody (VS-0225-XY38) | FC | Mouse IgG1, kappa |
| VS-0225-XY39 | CytoStream™ Mouse Anti-CD34 Recombinant Antibody (clone 8G12) | FC | Mouse IgG1, kappa |
| VS13-YC161 | CytoStream™ Rabbit Anti-CD34 Polyclonal Antibody (VS13-YC161) | WB, ELISA, IHC-P, IHC-Fr, FC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY362 | Anti-CD34 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1162 | Anti-Rat CD34 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1164 | Anti-Mouse CD34 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1161 | Anti-Human CD34 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC243 | Recombinant Anti-CD34 Vesicular Antibody, EV Displayed (VS-0425-YC243) | ELISA, FC, Inhib, Cell-uptake |
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