Human Anti-CD34 Recombinant Antibody (clone 2E10)
CAT#: FAMAB-1049CQ
2E10 is a recombinant human antibody that specifically targets CD34. The specificity of this antibody for human CD34 has been confirmed through western blot analysis. Furthermore, it recognizes a class III epitope.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36723/1751_G1_7_cr57fd220c70be0_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HUVEC TERT2, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174059-CD34
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Specifications
- Immunogen
- KG-1a cells, a cell line of human acute myeloid leukemia
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human CD34
- Species Reactivity
- Human
- Clone
- 2E10
- Applications
- WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot and Flow Cytometry.
Target
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Very accurate binding
In our lab, the Human Anti-CD34 Recombinant Antibody (clone 2E10) has changed everything. The extraordinary specificity and accuracy of binding to CD34-positive cells have given us unambiguous results. Our flow cytometry applications have become more efficient, and the quality of our data has increased.
Perfect for stem cell research
The Human Anti-CD34 Recombinant Antibody (clone 2E10) has been a valuable tool for our stem cell research. Our research depend on our ability to precisely detect and classify CD34-positive cells, which is made possible by the specificity. It's a dependable tool with accurate and consistent results.
Trusted performance
We have grown to trust the Human Anti-CD34 Recombinant Antibody (clone 2E10) that works in our laboratory. It is the preferred instrument for research involving the identification and isolation of CD34-positive cells since it continuously yields accurate and dependable results.
Q&As
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What possible difficulties might one encounter when utilizing this antibody?
A: Assuring the antibody's specificity and sensitivity under various experimental settings, streamlining the procedure for optimal effectiveness, and controlling sample preparation variability that may impact outcomes are a few of the difficulties. In order to overcome these obstacles, proper validation and controls are necessary.
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In what ways does the clone 2E10 further the field of stem cell research?
A: The clone 2E10 enables accurate stem and progenitor cell identification and isolation, hence supporting investigations into cell differentiation, regeneration, and therapeutic potential. This antibody aids in the development of novel cures and treatments as well as the advancement of our knowledge of stem cell biology.
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In repeated trials, how stable is the Human Anti-CD34 Recombinant Antibody (clone 2E10)?
A: Because the clone 2E10 is recombinant, meaning that quality and performance are guaranteed to be consistent between batches, it has demonstrated great reliability in repeated studies. For scientific study to produce repeatable data, this dependability is essential.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
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