Human Anti-CD72 Recombinant Antibody (clone BU40)
CAT#: FAMAB-1080CQ
This product is a recombinant human antibody that recognizes CD72. The antibody was purified by affinity chromatography. It can be used in Activ, Stim, FC.
Published Data
Gene Expression
WB
Figure 1 BU40 administration to Kasumi-1 cells induces the tyrosine phosphorylation of CD72 and association of CD72 and phospho-SHP-1.
After Kasumi-1 cells were incubated for the indicated time with control IgG or BU40, CD72 was immunoprecipitated with anti-CD72 (H-96), andvisualized with anti-phospho-tyrosine, anti-phospho-SHP-1, or anti-CD72. The cropped blots were shown, and the gels have been run under thesame conditions. Data are representative from three individual experiments.
Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.
FC
Figure 2 AML cell line Kasumi-1 express CD72.
Flow cytometry.
Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.
Apop
Figure 3 BU40 induces the cell death of Kasumi-1.
Kasumi-1 cells cultured in HEPES 10.04% BSA with vehicle, control IgG, BU40 or recombinant CD100 for 24 hours. The cells stained with propidium iodine(PI) without fixation, then analyzed using a FACS can flow cytometer. The cells incorporating PI were considered as the dead cells.
Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.
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Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44658/1373_H2_4_selected.jpg
Normal Tissue
Figure 2 Spleen
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2505/ihc_selected.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2505/6524_B_5_4.jpg
Normal Tissue
Figure 4 High expression in lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2505/6524_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, Karpas-707, REH, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137101-CD72
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- CD72
- Species Reactivity
- Human
- Clone
- BU40
- Applications
- WB, FC, Apop
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Apoptosis and Flow Cytometry.
Target
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Reliable Storage Specifications
I found the storage guidelines for this antibody very helpful. It stayed stable at -20°C and worked consistently, even after months.
Excellent Performance on Titration
This antibody performed exceptionally well when I performed a titration curve. It helped me determine the optimal concentration quickly.
Great Consistency
The consistency between different lots is satisfactory. Every batch has shown reliable and reproducible results in my experiments.
Q&As
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Is there an appropriate method for disposing of the antibody?
A: Dispose of biological materials in compliance with local laws and regulations to preserve safety and the environment.
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What is the average storage temperature for antibodies?
A: For long-term storage, most antibodies should be kept between -20°C and -80°C. For most applications, 4°C will do for brief periods. Observe the manufacturer's instructions at all times.
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How do I defrost the antibody?
A: Slowly thaw the antibody on ice to ensure stability and prevent deterioration or loss of effectiveness.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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|---|---|---|---|
| MOB-1916z | Mouse Anti-CD72 Recombinant Antibody (clone 24B5) | WB, FC, IHC, IP | Mouse IgG2a |
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| CAT | Product Name | Application | Type |
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| AGTO-G083P | Anti-CD72 immunotoxin J3-109 (IgG)-PAP | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
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| MOR-0578 | Hi-Affi™ Rabbit Anti-CD72 Recombinant Antibody (clone DS578AB) | ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0062CQ-S(P) | Human Anti-CD72 Recombinant Antibody (clone sc02-004); scFv Fragment | IHC | Human scFv |
| HPAB-0063CQ-S(P) | Human Anti-CD72 Recombinant Antibody (clone sc02-025); scFv Fragment | IHC | Human scFv |
| HPAB-2053-FY-S(P) | Human Anti-CD72 Recombinant Antibody (clone II-2); scFv Fragment | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0062CQ-F(E) | Human Anti-CD72 Recombinant Antibody (clone sc02-004); Fab Fragment | IHC | Human Fab |
| HPAB-0063CQ-F(E) | Human Anti-CD72 Recombinant Antibody (clone sc02-025); Fab fragment | IHC | Human Fab |
| HPAB-2053-FY-F(E) | Human Anti-CD72 Recombinant Antibody (clone II-2); Fab Fragment | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC506 | Recombinant Anti-CD72 Vesicular Antibody, EV Displayed (VS-0425-YC506) | ELISA, FC, Cell-uptake |
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|---|---|---|---|
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