Human Anti-CD72 Recombinant Antibody (clone BU40) (CAT#: FAMAB-1080CQ)
This product is a recombinant human antibody that recognizes CD72. The antibody was purified by affinity chromatography. It can be used in Activ, Stim, FC.
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WB
Figure 1 BU40 administration to Kasumi-1 cells induces the tyrosine phosphorylation of CD72 and association of CD72 and phospho-SHP-1.
After Kasumi-1 cells were incubated for the indicated time with control IgG or BU40, CD72 was immunoprecipitated with anti-CD72 (H-96), andvisualized with anti-phospho-tyrosine, anti-phospho-SHP-1, or anti-CD72. The cropped blots were shown, and the gels have been run under thesame conditions. Data are representative from three individual experiments.
Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.
FC
Figure 2 AML cell line Kasumi-1 express CD72.
Flow cytometry.
Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.
Apop
Figure 3 BU40 induces the cell death of Kasumi-1.
Kasumi-1 cells cultured in HEPES 10.04% BSA with vehicle, control IgG, BU40 or recombinant CD100 for 24 hours. The cells stained with propidium iodine(PI) without fixation, then analyzed using a FACS can flow cytometer. The cells incorporating PI were considered as the dead cells.
Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- CD72
- Species Reactivity
- Human
- Clone
- BU40
- Applications
- WB, FC, Apop
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Apoptosis and Flow Cytometry.
Target
- Alternative Names
- CD72 Molecule; CD72 Antigen; Lyb-2; B-Cell Differentiation Antigen CD72; CD72b; LYB2
- Gene ID
- 971
- UniProt ID
- P21854
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1916z | Mouse Anti-CD72 Recombinant Antibody (clone 24B5) | WB, FC, IHC, IP | Mouse IgG2a |
| MOB-0273MC | Human Anti-CD72 Recombinant Antibody (clone YC015) | ELISA, WB, FuncS | |
| MOB-0274MC | Human Anti-CD72 Recombinant Antibody (clone YC016); scFv Fragment | ELISA, WB, FuncS | Human scFv |
| HPAB-0062CQ | Human Anti-CD72 Recombinant Antibody (clone sc02-004) | IHC | Human IgG1, κ |
| HPAB-2053-FY | Human Anti-CD72 Recombinant Antibody (clone II-2) | FC | Human IgG1 |
Customer Reviews and Q&As
Customer Review
Q&As
Reliable Storage Specifications
I found the storage guidelines for this antibody very helpful. It stayed stable at -20°C and worked consistently, even after months.
Excellent Performance on Titration
This antibody performed exceptionally well when I performed a titration curve. It helped me determine the optimal concentration quickly.
Great Consistency
The consistency between different lots is satisfactory. Every batch has shown reliable and reproducible results in my experiments.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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