Human Anti-CD72 Recombinant Antibody (clone BU40) (CAT#: FAMAB-1080CQ)

Figure 1 BU40 administration to Kasumi-1 cells induces the tyrosine phosphorylation of CD72 and association of CD72 and phospho-SHP-1.

After Kasumi-1 cells were incubated for the indicated time with control IgG or BU40, CD72 was immunoprecipitated with anti-CD72 (H-96), andvisualized with anti-phospho-tyrosine, anti-phospho-SHP-1, or anti-CD72. The cropped blots were shown, and the gels have been run under thesame conditions. Data are representative from three individual experiments.

Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.

Figure 2 AML cell line Kasumi-1 express CD72.

Flow cytometry.

Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.

Figure 3 BU40 induces the cell death of Kasumi-1.

Kasumi-1 cells cultured in HEPES 10.04% BSA with vehicle, control IgG, BU40 or recombinant CD100 for 24 hours. The cells stained with propidium iodine(PI) without fixation, then analyzed using a FACS can flow cytometer. The cells incorporating PI were considered as the dead cells.

Kataoka, T. R., Kumanogoh, A., Hirata, M., Moriyoshi, K., Ueshima, C., Kawahara, M., ... & Haga, H. (2013). CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 3(1), 1-5.