Mouse Anti-CD99 Recombinant Antibody (clone PT1849) (CAT#: VS3-CJ650)
This product is a mouse antibody that recognizes human CD99.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
(Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
Figure 5 Pancreas
(Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
(Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
Specifications
- Immunogen
- Synthesized peptide derived from human CD99
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD99
- Species Reactivity
- Human
- Clone
- PT1849
- Applications
- IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- MIC2; HBA71; MIC2X; MIC2Y; MSK5X
- Gene ID
- 4267
- UniProt ID
- P14209
- Sequence Similarities
- Belongs to the CD99 family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Extensively O-glycosylated.
- Protein Refseq
- NP_001116370.1; NP_001308296.1; NP_001308297.1
- Function
- Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1118z | Mouse Anti-CD99 Recombinant Antibody (clone 40G11) | WB, FC, IHC | Mouse IgG1 |
| MRO-0323-CN | Rabbit Anti-CD99 Recombinant Antibody (clone JF0991) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0139J | Mouse Anti-CD99 Recombinant Antibody (ZG-0139J) | IHC-P, IF | Mouse IgG |
| ZG-0140J | Mouse Anti-CD99 Recombinant Antibody (ZG-0140J) | IHC-P, IF | Mouse IgG |
| VS-0723-WK129 | Rabbit Anti-CD99 Recombinant Antibody (VS-0723-WK129) | ELISA, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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