Mouse Anti-CD99 Recombinant Antibody (clone PT1849)
CAT#: VS3-CJ650
This product is a mouse antibody that recognizes human CD99.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
Normal Tissue
Figure 5 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
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Specifications
- Immunogen
- Synthesized peptide derived from human CD99
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD99
- Species Reactivity
- Human
- Clone
- PT1849
- Applications
- IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- MIC2; HBA71; MIC2X; MIC2Y; MSK5X
- Gene ID
- 4267
- UniProt ID
- P14209
- Sequence Similarities
- Belongs to the CD99 family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Extensively O-glycosylated.
- Protein Refseq
- NP_001116370.1; NP_001308296.1; NP_001308297.1
- Function
- Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
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| TAB-401LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-401LC-S(P)) | FC, ELISA | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-402LC | Human Anti-CD99 Recombinant Antibody (TAB-402LC) | ELISA, FC | Human IgG |
| TAB-402LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-402LC-S(P)) | ELISA, FC | Human scFv |
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