CytoStream™ Rabbit Anti-CD99 Recombinant Antibody (VS13-YC180) (CAT#: VS13-YC180)
The CytoStream™ Rabbit Anti-CD99 Recombinant Antibody is specific for human CD99. CD99 is linked to the Xg blood group and associated with diseases like Ewing's Sarcoma, participating in pathways involving platelet cytosolic Ca2+ and vascular interactions. The anti-CD99 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
(Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
Figure 5 Pancreas
(Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
(Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
Specifications
- Immunogen
- Recombinant human CD99, aa 1-130.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD99
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
- Related Disease
- Extraosseous Ewing's Sarcoma and Ewing Sarcoma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- CD99 Molecule (Xg Blood Group); CD99 Antigen; Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13; T-Cell Surface Glycoprotein E2; E2 Antigen; MIC2X; MIC2Y; MIC2; Antigen Identified By Monoclonal 12E7, Y Homolog; MIC2 (Monoclonal Antibody 12E7);
- Gene ID
- 4267
- UniProt ID
- P14209
- Long Name
- CD99 Molecule (Xg Blood Group)
- Sequence Similarities
- Belongs to the CD99 family.
- Cellular Localization
- Cell membrane
- Protein Refseq
- NP_001116370.1; NP_001308296.1; NP_001308297.1
- Function
- Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Chimeric Antibody Products
Humanized Antibody Products
Human Antibody Products
IgG Antibody Products
Rabbit Monoclonal Antibody Products
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-400LC | Human Anti-CD99 Recombinant Antibody (TAB-400LC) | FC, ELISA | Chimeric (Mouse/Human) IgG1 |
| TAB-400LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-400LC-S(P)) | FC, ELISA | Human scFv |
| TAB-400LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-400LC-F(E)) | FC, ELISA | Chimeric (Mouse/Human) Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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