Rabbit Anti-CD99 Recombinant Antibody (clone R06-8G3)
CAT#: VS3-FY1678
This is a rabbit recombinant monoclonal antibody, which binds to human CD99. It cross-reacts with mouse. The R06-8G3 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
Normal Tissue
Figure 5 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
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Specifications
- Immunogen
- Synthetic peptide of human CD99
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CD99
- Species Reactivity
- Human, Mouse
- Clone
- R06-8G3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 19 kDa, while the observed molecular weight stands at 30 kDa.
- Related Disease
- Extraosseous Ewing's Sarcoma and Ewing Sarcoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- CD99 Molecule (Xg Blood Group); CD99 Antigen; Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13; T-Cell Surface Glycoprotein E2; E2 Antigen; MIC2X; MIC2Y; MIC2; Antigen Identified By Monoclonal 12E7, Y Homolog; MIC2 (Monoclonal Antibody 12E7)
- Gene ID
- 4267
- UniProt ID
- P14209
- Sequence Similarities
- Belongs to the CD99 family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Extensively O-glycosylated.
- Protein Refseq
- NP_001116370.1; NP_001308296.1; NP_001308297.1
- Function
- Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1118z | Mouse Anti-CD99 Recombinant Antibody (clone 40G11) | WB, FC, IHC | Mouse IgG1 |
| ZG-0601J | Rabbit Anti-CD99 Recombinant Antibody (clone 9A5) | ELISA, WB, IHC, FC | Rabbit IgG |
| VS3-FY327 | Recombinant Mouse Anti-CD99 Antibody (clone 6C7-2G6-2C5) | IHC-P | Mouse IgG |
| VS3-FY328 | Recombinant Rabbit Anti-CD99 Antibody (clone R02-4H1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-400LC | Human Anti-CD99 Recombinant Antibody (TAB-400LC) | FC, ELISA | Chimeric (Mouse/Human) IgG1 |
| TAB-400LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-400LC-S(P)) | FC, ELISA | Human scFv |
| TAB-400LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-400LC-F(E)) | FC, ELISA | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-401LC | Human Anti-CD99 Recombinant Antibody (TAB-401LC) | FC, ELISA, ADCC | Human IgG1 |
| TAB-401LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-401LC-S(P)) | FC, ELISA | Human scFv |
| TAB-401LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-401LC-F(E)) | FC, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-402LC | Human Anti-CD99 Recombinant Antibody (TAB-402LC) | ELISA, FC | Human IgG |
| TAB-402LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-402LC-S(P)) | ELISA, FC | Human scFv |
| TAB-402LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-402LC-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0595 | Hi-Affi™ Rabbit Anti-CD99 Recombinant Antibody (clone DS595AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY209 | Human Anti-CD99 scFv-Fc Chimera (VS-0325-FY209) | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC180 | CytoStream™ Rabbit Anti-CD99 Recombinant Antibody (VS13-YC180) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC162 | Recombinant Anti-CD99 Vesicular Antibody, EV Displayed (VS-0425-YC162) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1250 | Anti-CD99 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1251 | Anti-Rat CD99 Immunohistochemistry Kit | IHC |
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