Rabbit Anti-CDC27 Recombinant Antibody (VS3-CJ864)
CAT#: VS3-CJ864
This product is a rabbit antibody that recognizes human, mouse, and rat CDC27.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28129/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65835/1807_D10_31_selected.jpg
Normal Tissue
Figure 3 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28129/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/ihc_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_7_8.jpg
Normal Tissue
Figure 8 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35570_B_4_1.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_7_3.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004897-CDC27
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat CDC27
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot.
Target
- Alternative Names
- APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
- Gene ID
- 996
- UniProt ID
- P30260
- Sequence Similarities
- Belongs to the APC3/CDC27 family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Phosphorylated. Phosphorylation on Ser-426 and Thr-446 oCcurs specifically during mitosis.
- Protein Refseq
- NP_001107563.1; NP_001247.3
- Function
- Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDC27"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0602 | Hi-Affi™ Rabbit Anti-CDC27 Recombinant Antibody (clone DS602AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0326-CN | Rabbit Anti-CDC27 Recombinant Antibody (clone CBACN-127) | WB | Rabbit IgG |
| ZG-0401F | Mouse Anti-CDC27 Recombinant Antibody (ZG-0401F) | WB, IHC, ELISA | Mouse IgG |
| VS3-QX201 | Mouse Anti-CDC27 Recombinant Antibody (clone 5C12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-HM662 | Mouse Anti-CDC27 Recombinant Antibody (clone CBL132HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| VS13-YC185 | Rabbit Anti-CDC27 Recombinant Antibody (VS13-YC185) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY383 | Anti-CDC27 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1260 | Anti-Frog CDC27 Immunohistochemistry Kit | IHC |
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