Mouse Anti-CDC27 Recombinant Antibody (ZG-0401F)
CAT#: ZG-0401F
This product is a mouse antibody that recognizes CDC27.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CDC27 Recombinant Antibody (ZG-0401F) in WB.
Western blot analysis was performed using CDC27 monoclonal antibody against CDC27-hIgGFc transfected HEK293 cells.
IHC
Figure 2 Mouse Anti-CDC27 Recombinant Antibody (ZG-0401F) in IHC.
The CDC27 monoclonal antibody was used for immunohistochemical analysis of paraffin-embedded lung cancer tissue (left) and colon cancer tissue (right) by DAB staining.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28129/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65835/1807_D10_31_selected.jpg
Normal Tissue
Figure 3 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28129/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/ihc_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_7_8.jpg
Normal Tissue
Figure 8 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35570_B_4_1.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16315/35517_A_7_3.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004897-CDC27
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Specifications
- Immunogen
- Recombinant fragment of human CDC27 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDC27
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- Cell Division Cycle 27; Anaphase Promoting Complex Subunit 3; D0S1430E, D17S978E; D0S1430E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; CDC27; ANAPC3; D0S1430E; D17S978E; Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC
- Gene ID
- 996
- UniProt ID
- P30260
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CDC27"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2786z | Mouse Anti-CDC27 Recombinant Antibody (clone 26G11) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-0326-CN | Rabbit Anti-CDC27 Recombinant Antibody (clone CBACN-127) | WB | Rabbit IgG |
| VS3-CJ864 | Rabbit Anti-CDC27 Recombinant Antibody (VS3-CJ864) | WB | Rabbit IgG |
| VS7-HM662 | Mouse Anti-CDC27 Recombinant Antibody (clone CBL132HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| VS13-YC185 | Rabbit Anti-CDC27 Recombinant Antibody (VS13-YC185) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0602 | Hi-Affi™ Rabbit Anti-CDC27 Recombinant Antibody (clone DS602AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY383 | Anti-CDC27 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1260 | Anti-Frog CDC27 Immunohistochemistry Kit | IHC |
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