AbPlus™ Anti-CDK4 Magnetic Beads (VS-0724-YC569) (CAT#: VS-0724-YC569)
The AbPlus Anti-CDK4 Magnetic Beads (VS-0724-YC569) is an innovative affinity resin which is bound with anti-CDK4 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of CDK4 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli, nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6024/1704_B1_1_cr5811e29350250_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli, nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6024/1704_B1_1_cr5811e29350250_selected.jpg

Figure 2 IHC staining of human ovary
(Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_4_7_selected.jpg
(Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_4_7_selected.jpg

Figure 3 IHC staining of human endometrium
(Immunohistochemical staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69405/163411_B_5_3_selected.jpg
(Immunohistochemical staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69405/163411_B_5_3_selected.jpg

Figure 4 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Low Intensity: Moderate Quantity: <25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_7_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Low Intensity: Moderate Quantity: <25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_7_3.jpg

Figure 5 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_5_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_5_6.jpg

Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_9_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_9_8.jpg

Figure 7 RNA cell line category: Group enriched (HBEC3-KT, LHCN-M2, RH-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135446-CDK4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135446-CDK4
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-CDK4 specific antibody
- Target
- CDK4
- Immunogen
- Recombinant human CDK4, aa 1-303.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Alternative Names
- Cyclin Dependent Kinase 4; Cell Division Protein Kinase 4; EC 2.7.11.22; PSK-J3; Cyclin-Dependent Kinase 4; EC 2.7.11; CMM3;
- Gene ID
- 1019
- UniProt ID
- P11802
Related Resources
Downloads
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CAT | Product Name | Application | Type |
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MOB-0519CT | Recombinant Mouse anti-Human CDK4 Monoclonal antibody (EML005) | ICC/IF, WB | |
ZG-0794F | Mouse Anti-CDK4 Recombinant Antibody (clone DCS31.2) | WB, IP, IF | Mouse IgG1 |
VS3-CJ598 | Mouse Anti-CDK4 Recombinant Antibody (VS3-CJ598) | WB, IHC | Mouse IgG2a, κ |
VS3-CJ599 | Rabbit Anti-CDK4 Recombinant Antibody (VS3-CJ599) | WB, ICC, IF, IHC, FC | Rabbit IgG |
VS4-WK100 | Mouse Anti-CDK4 Recombinant Antibody (VS4-WK100) | ELISA, WB, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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