Mouse Anti-CDK4 Recombinant Antibody (clone 23A2)
CAT#: MOB-2055z
This product is a mouse antibody that recognizes human CDK4. It can be used in the following applications: WB, ICC, IF, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli, nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6024/1704_B1_1_cr5811e29350250_selected.jpg
Normal Tissue
Figure 2 IHC staining of human ovary
Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_4_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69405/163411_B_5_3_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Low Intensity: Moderate Quantity: <25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (HBEC3-KT, LHCN-M2, RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135446-CDK4
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Specifications
- Immunogen
- Human Cyclin-Dependent Kinase 4
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDK4
- Species Reactivity
- Human
- Clone
- 23A2
- Applications
- WB, ICC, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- CDK4; EC 2.7.11.22; Cell Division Protein Kinase 4; CMM3; Cyclin-Dependent Kinase 4; EC 2.7.11; PSK-J3
- UniProt ID
- P11802
REVIEWS AND Q&AS
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| BRD-0113MZ | Chicken Anti-CDK4 Polyclonal IgY | WB | Chicken antibody |
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| MOR-0630 | Hi-Affi™ Rabbit Anti-CDK4 Recombinant Antibody (clone DS630AB) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| ZG-0612J | Rabbit Anti-CDK4 Recombinant Antibody (clone 8F2) | ELISA, WB, IP | Rabbit IgG |
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| VS3-FY346 | Recombinant Rabbit Anti-CDK4 Antibody (clone R04-4I5) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY347 | Recombinant Rabbit Anti-CDK4 Antibody (clone R04-8I5) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC569 | AbPlus™ Anti-CDK4 Magnetic Beads (VS-0724-YC569) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY404 | Anti-CDK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1317 | Anti-Mouse CDK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1316 | Anti-Human CDK4 Immunohistochemistry Kit | IHC |
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