Recombinant Mouse anti-Human CDK4 Monoclonal antibody (EML005)
CAT#: MOB-0519CT
Recombinant Mouse monoclonal antibody to Human CDK4, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli, nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6024/1704_B1_1_cr5811e29350250_selected.jpg
Normal Tissue
Figure 2 IHC staining of human ovary
Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_4_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69405/163411_B_5_3_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Low Intensity: Moderate Quantity: <25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13116/163415_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (HBEC3-KT, LHCN-M2, RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135446-CDK4
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Specifications
- Immunogen
- Recombinant fragment: KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE, corresponding to amino acids 211-304 of Human Cdk4
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML005
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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