Rabbit Anti-CDKN1A Recombinant Antibody (clone R04-4H1) (CAT#: VS3-XY3308)
This is a recombinant rabbit antibody that specifically recognizes human CDKN1A (Cyclin dependent kinase inhibitor 1A), which plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. It also has cross-reactivity against mouse or rat CDKN1A. It has been reported for use in Western Blot, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot and Immunofluorescence is 1/500-1/1000 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5946/1541_C12_6_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5946/1541_C12_6_selected.jpg
Figure 2 IHC staining of human cervix, uterine
(Immunohistochemical staining of human cervix, uterine shows nuclear positivity in squamous cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/156817_B_8_3_selected.jpg
(Immunohistochemical staining of human cervix, uterine shows nuclear positivity in squamous cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/156817_B_8_3_selected.jpg
Figure 3 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69401/155899_A_6_5_selected.jpg
(Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69401/155899_A_6_5_selected.jpg
Figure 4 Colon
(Glandular cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_7_3.jpg
(Glandular cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_7_3.jpg
Figure 5 Pancreas
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_1_3.jpg
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_1_3.jpg
Figure 6 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_5_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64/159107_A_5_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, HSkMC, hTCEpi, LHCN-M2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124762-CDKN1A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124762-CDKN1A
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CDKN1A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4H1
- Applications
- WB, ICC, IF
- Related Disease
- Bladder Cancer; Multiple Endocrine Neoplasia, Type I
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
- Gene ID
- 1026
- UniProt ID
- P38936
- Long Name
- Cyclin dependent kinase inhibitor 1A
- Sequence Similarities
- Belongs to the CDI family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Ubiquitinated by MKRN1.
- Protein Refseq
- NP_000380.1; NP_001207706.1; NP_001207707.1
- Function
- May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-190 | Recombinant Anti-human CDKN1A Antibody | ELISA, WB, Neut, FuncS | IgG |
| MHH-190 | Recombinant Human Anti-human CDKN1A Antibody | ELISA, IF, FuncS | IgG |
| MOB-2113MZ | Recombinant Mouse Anti-Human CDKN1A Antibody (clone FB20) | FC, ICC, IHC-Fr, IHC-P, IP, WB | Mouse antibody |
| ZG-0797F | Mouse Anti-CDKN1A Recombinant Antibody (clone DCS-60) | WB, IP, IHC | Mouse IgG2a |
| VS3-FY352 | Recombinant Rabbit Anti-CDKN1A Antibody (clone R07-3K5) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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