Mouse Anti-CDKN2A Monoclonal Antibody (clone 1E12E10) (CAT#: VS7-0425-WR532)
This product is a mouse IgG1 antibody that binds to human CDKN2A. The antibody 1E12E10 can be used for IHC assay. It cross reacts with rat and mouse.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47838/979_F9_4_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47838/979_F9_4_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/93/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/93/ihc_selected.jpg
Figure 3 Tonsil
(High expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_4_8_val_selected.jpg
(High expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_4_8_val_selected.jpg
Figure 4 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18232/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18232/ihc_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_8.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HEK93, HeLa, SK-MEL-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147889-CDKN2A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147889-CDKN2A
Specifications
- Immunogen
- Recombinant human CDKN2A fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CDKN2A
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 1E12E10
- Molecule Class
- INK4 Family Of Cyclin-Dependent Kinase Inhibitors
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Melanoma, cutaneous malignant 2 (CMM2); Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC); Melanoma-astrocytoma syndrome (MASTS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CDKN2A; CDK4I; P14ARF; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase Inhibitor 2A; CDK4 Inhibitor P16-INK4; P16-INK4A; P19; TP16; P16-INK4; P14; MLM; MTS-1; P19ARF; P16INK4A
- Gene ID
- 1029
- UniProt ID
- P42771
- Long Name
- Cyclin Dependent Kinase Inhibitor 2A
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000068.1
- Function
- Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2201MZ | Recombinant Mouse Anti-Human CDKN2A Antibody (clone 25Q03 (tbnf bt EDT-350)) | ICC/IF, IP, WB | Mouse antibody |
| ZG-0333C | Mouse Anti-CDKN2A Recombinant Antibody (ZG-0333C) | WB, IHC, ELISA | Mouse IgG |
| ZG-0334C | Mouse Anti-CDKN2A Recombinant Antibody (ZG-0334C) | WB, IHC, ELISA | Mouse IgG |
| ZG-0335C | Mouse Anti-CDKN2A Recombinant Antibody (ZG-0335C) | WB, IHC, ELISA | Mouse IgG |
| ZG-0579F | Mouse Anti-CDKN2A Recombinant Antibody (clone DCS-50) | IP, IHC, WB | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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