Mouse Anti-CDKN2A Recombinant Antibody (ZG-0333C)
CAT#: ZG-0333C
This product is a mouse antibody that recognizes CDKN2A. It can be used in a wide variety of applications, such as: WB, IHC, ELISA.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CDKN2A Antibody (ZG-0333C) in WB
Western Blot analysis using p16 monoclonal antibody against truncated p16 recombinant protein.
IHC
Figure 2 Mouse Anti-CDKN2A Antibody (ZG-0333C) in IHC
Immunohistochemistry analysis of paraffin-embedded human lung adenocarcinoma (A), esophageal squamous cell carcinoma (B), hepatic cell carcinoma (C), thyroid tumor (D), breast adenofibroma (E), breast infiltrating ductal carcinoma (F), normal cerebrum tissue (G), normal colon tissue (H), normal esophageal tissue (I) using P16 monoclonal antibody followed with DAB staining.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47838/979_F9_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/93/ihc_selected.jpg
Normal Tissue
Figure 3 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_4_8_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18232/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HEK93, HeLa, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147889-CDKN2A
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Specifications
- Immunogen
- Purified recombinant fragment of p16 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human p16
- Species Reactivity
- Human
- Applications
- WB, IHC, ELISA
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- WB 1:500-1:2000. IHC 1:200-1:1000. ELISA 1:10000.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-2201MZ | Recombinant Mouse Anti-Human CDKN2A Antibody (clone 25Q03 (tbnf bt EDT-350)) | ICC, IF, IP, WB | Mouse antibody |
| ZG-0394U | Mouse Anti-CDKN2A Recombinant Antibody (clone R15-A) | IHC-P | Mouse IgG |
| VS3-XY481 | Mouse Anti-CDKN2A Recombinant Antibody (clone 1D7D2) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY2607 | Rabbit Anti-CDKN2A Recombinant Antibody (VS3-FY2607) | WB, ICC, IP | Rabbit IgG |
| VS7-HM687 | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL148HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| BRD-0116MZ | Chicken Anti-CDKN2A Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0642 | Hi-Affi™ Rabbit Anti-CDKN2A Recombinant Antibody (clone DS642AB) | FC, ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0877LY-S(P) | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015); scfv Fragment | IHC, WB | Mouse scfv |
| HPAB-M0037-YC-S(P) | Mouse Anti-CDKN2A Recombinant Antibody (clone P1); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0877LY-F(E) | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015); Fab Fragment | IHC, WB | Mouse Fab |
| HPAB-M0037-YC-F(E) | Mouse Anti-CDKN2A Recombinant Antibody (clone P1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1355 | AbPlus™ Anti-CDKN2A Magnetic Beads (VS-0724-YC1355) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY414 | Anti-CDKN2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1341 | Anti-Rat CDKN2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1340 | Anti-Human CDKN2A Immunohistochemistry Kit | IHC |
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