Mouse Anti-CHD4 Recombinant Antibody (clone 5F1-A11-C9)
CAT#: VS3-QX234
This product is a mouse antibody that recognizes CHD4. The antibody can be used in applications: WB, IP.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/572_F7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/564_F7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/587_F7_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111642-CHD4
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Specifications
- Immunogen
- Purified recombinant human CHD4 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- CHD4
- Species Reactivity
- Human
- Clone
- 5F1-A11-C9
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CHD4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-302 | Human Anti-CHD4 Recombinant Antibody (clone D323); Fab Fragment | IP, IF, ELISA, IP-MS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-303 | Human Anti-CHD4 Recombinant Antibody (clone D406) | IF, ELISA, IP-MS | Human IgG |
| MOB-0487CT | Recombinant Mouse anti-Human CHD4 Monoclonal antibody (EML045) | FC, IHC-P, WB | |
| MRO-0358-CN | Rabbit Anti-CHD4 Recombinant Antibody (clone JA33-40) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0417F | Mouse Anti-CHD4 Recombinant Antibody (ZG-0417F) | WB | Mouse IgG |
| VS4-WK107 | Mouse Anti-CHD4 Recombinant Antibody (VS4-WK107) | ELISA, WB, IP | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0686 | Hi-Affi™ Rabbit Anti-CHD4 Recombinant Antibody (clone DS686AB) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY64 | AbPlus™ Anti-CHD4 Magnetic Beads (D406) | IP-MS, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY444 | Anti-CHD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1427 | Anti-Mouse CHD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1426 | Anti-Human CHD4 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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