Human Anti-CHD4 Recombinant Antibody (clone D323); Fab Fragment

Name: Human Anti-CHD4 Recombinant Antibody (clone D323); Fab Fragment
SKU: DrMAB-302
Rating: 5 (1 reviews)

CAT#: DrMAB-302

Recombinant Human Antibody binds selectively to Human CHD4, expressed in E. coli.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IHC staining of human colon

Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/572_F7_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/564_F7_1_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/587_F7_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_B_7_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_9_3.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_4_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_7_8.jpg

RNA Expression Figure 10 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111642-CHD4

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Specifications

Host Species
Human

Type
Human Fab

Specificity
Human CHD4

Species Reactivity
Human

Clone
D323

Applications
IP, IF, ELISA, IP-MS

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CHD4; chromodomain helicase DNA binding protein 4; CHD-4; Mi-2b; Mi2-BETA; chromodomain-helicase-DNA-binding protein 4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein

Gene ID
1108

UniProt ID
Q14839

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - DrMAB-302.pdf

MSDS

MSDS - DrMAB-302.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "CHD4"

Select a product category from the dropdown menu below to view related products.

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DrMAB-303	Human Anti-CHD4 Recombinant Antibody (clone D406)	IF, ELISA, IP-MS	Human IgG
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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