Mouse Anti-CHD4 Recombinant Antibody (ZG-0417F)
CAT#: ZG-0417F
This product is a mouse antibody that recognizes CHD4.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CHD4 Recombinant Antibody (ZG-0417F) in WB.
Western blot detection of CHD4 in K562, HelaNE, HL-60, MOLT-4, Jurkat, and 293 cell lysates was performed using CHD4 mouse mAb (1:1000 dilution). Predicted band size: 260KDa. Observe the band size: 260KDa.
IP
Figure 2 Mouse Anti-CHD4 Recombinant Antibody (ZG-0417F) in IP.
The CHD4 mouse mAb was used for immunoprecipitation analysis of K562 cell lysates.
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/572_F7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/564_F7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/587_F7_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12008/51702_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111642-CHD4
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Specifications
- Immunogen
- Recombinant human CHD4 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CHD4
- Species Reactivity
- Human
- Applications
- Western Blot
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Target
- Alternative Names
- Chromodomain Helicase DNA Binding Protein 4; Mi-2 Autoantigen 218 KDa Protein; ATP-Dependent Helicase CHD4; Mi2-BETA; CHD-4; Chromodomain-Helicase-DNA-Binding Protein 4; ATP dependent helicase CHD4; ATP-dependent helicase CHD4; CHD 4; CHD-4; CHD4; CHD4_HUMAN; Chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; DKFZp686E06161; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi-2 autoantigen 218 kDa protein; Mi2 beta; Mi2-beta.
- Gene ID
- 1108
- UniProt ID
- Q14839
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| DrMAB-302 | Human Anti-CHD4 Recombinant Antibody (clone D323); Fab Fragment | IP, IF, ELISA, IP-MS | Human Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-0686 | Hi-Affi™ Rabbit Anti-CHD4 Recombinant Antibody (clone DS686AB) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0325-XY444 | Anti-CHD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1427 | Anti-Mouse CHD4 Immunohistochemistry Kit | IHC | |
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