Mouse Anti-CHRM2 Recombinant Antibody (VS3-CJ487) (CAT#: VS3-CJ487)

• Immunogen
• Recombinant protein

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human, Mouse CHRM2

• Species Reactivity
• Human, Mouse

• Applications
• WB, IF, IHC-P, FC

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000), Immunofluorescence (1:25), Immunohistochemistry-Parraffin (1:25), Flow Cytometry (1:25).

• Alternative Names
• HM2

• Gene ID
• 1129

• UniProt ID
• P08172

• Sequence Similarities
• Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

• Cellular Localization
• Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

• Post Translation Modifications
• Phosphorylated in response to agonist treatment.

• Protein Refseq
• NP_000730.1; NP_001006627.1; NP_001006628.1

• Function
• The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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